Sökning: "Matthew Delisa"

Hittade 2 avhandlingar innehållade orden Matthew Delisa.

  1. 1. Membrane and secretory protein production in Escherichia coli

    Författare :Alexandros Karyolaimos; Jan-Willem de Gier; Matthew DeLisa; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; recombinant protein; tunable protein production; cytoplasmic membrane; periplasm; signal peptide; force profile analysis; co-translational folding; Biochemistry; biokemi;

    Sammanfattning : Escherichia coli (E. coli) is the most widely used bacterium for the production of recombinant proteins. However, the production of proteins in its cytoplasmic membrane or periplasm is challenging. Therefore, the aim of this doctoral thesis was to develop setups for enhancing the production of membrane and secretory proteins in E. LÄS MER

  3. 2. Intracellular systems for characterization and engineering of proteases and their substrates

    Författare :George Kostallas; Stefan Ståhl; Patrik Samuelson; Matthew DeLisa; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; proteases; flow cytometry; GFP; CAT; protein engineering; ssrA; ClpXP; tobacco etch virus; TEV; site-specific; high-throughput; selection; proteolysis; libraries; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : Over the years, the view on proteases as relatively non-specific protein degradation enzymes, mainly involved in food digestion and intracellular protein turnover, has shifted and they are now recognized as key regulators of many biological processes that determine the fate of a cell. Besides their biological role, proteases have emerged as important tools in various biotechnical, industrial and medical applications. LÄS MER