Sökning: "Massively parallel sequencing"

Visar resultat 16 - 20 av 36 avhandlingar innehållade orden Massively parallel sequencing.

1. 16. Next generation molecular diagnostics using ultrasensitive sequencing

   Författare :Stefan Filges; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Liquid biopsy; cell-free DNA; circulating tumor DNA; molecular diagnostics; next-generation sequencing; unique molecular identifiers; melanoma;

   Sammanfattning : Massively parallel sequencing enables the exploration of the genetic heterogeneity within microbial, viral and tumor cell populations. Detecting circulating tumor DNA in blood and other body fluids has the potential to revolutionize molecular diagnostics. LÄS MER

3. 17. Somatic Mutations in Breast Cancer Genomes : Discovery and Validation of Breast Cancer Genes

   Författare :Xiang Jiao; Tobias Sjöblom; Anne-Lise Børresen-Dale; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; breast cancer; cancer gene; pathway; somatic mutation; structural alteration; sequencing; whole genome amplification; Genetics; Genetik; Medical Genetics; Medicinsk genetik;

   Sammanfattning : Breast cancer is the most common cancer in women worldwide. However, the genetic alterations that lead to breast cancer are not fully understood. This thesis aims to identify novel genes of potential mechanistic, diagnostic or therapeutic interest in breast cancers by mutational analysis and whole-genome sequencing. LÄS MER

4. 18. Towards spatial host-microbiome profiling

   Författare :Britta Lötstedt; Joakim Lundeberg; Petri Auvinen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; 16S sequencing; RNA sequencing; spatially resolved transcriptomics; antibody-based measurements; Bioteknologi; Biotechnology;

   Sammanfattning : Sequencing technologies and applications have pushed the limits and enabled novel studies of biological mechanisms, evolutionary relationships and communication networks between cells. The technical developments leading to single cell RNA-sequencing have enabled detection of rare cell populations while spatial resolution added insights into larger biological environments, like tissues and organs. LÄS MER

5. 19. Advances in DNA Detection

   Författare :Yajing Song; Olof Emanuelsson; Jonas Tegenfeldt; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA detection; active filter paper; visual detection; throughput; fluorescence; superparamagnetic beads; rare DNA; Q-PCR; chemical fragmentation; DNA library preparation; massively parallel sequencing; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : DNA detection technologies have an increasing importance in our everyday lives, with applications ranging from microbial diagnostics to forensic analysis, food safety evaluation, and environmental monitoring. Currently, as the associated costs decrease, DNA diagnostic techniques are routinely used in research laboratories, in clinical and forensic practice. LÄS MER

7. 20. Applications of Four-Colour Fluorescent Primer Extension Technology for SNP Analysis and Discovery

   Författare :Annika Ahlford; Ann-Christine Syvänen; Mats Nilsson; David Schwartz; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single nucleotide polymorphism; Genotyping; Massively parallel sequencing; Gene mapping; Lab-on-a-chip; Medical genetics; Medicinsk genetik; Molecular biology; Molekylärbiologi; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Studies on genetic variation can reveal effects on traits and disease, both in humans and in model organisms. Good technology for the analysis of DNA sequence variations is critical. Currently the development towards assays for large-scale and parallel DNA sequencing and genotyping is progressing rapidly. LÄS MER