Sökning: "Magnetic bead separation"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden Magnetic bead separation.

  1. 1. Magnetic bead-based isolation of biological therapeutic modalities

    Författare :Nils A. Brechmann; Véronique Chotteau; Nigel Titchener-Hooker; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Magnetic bead separation; Magnetic beads; Protein A; Protein G; Negative cell isolation; Monoclonal antibody; induced pluripotent stem cells; human mesenchymal stem cells; Allogenic cell therapy; Biomanufacturing; cell clarification; Biotechnology; Bioteknologi;

    Sammanfattning : Biopharmaceutical modalities, such as monoclonal antibodies or the less established cell therapies, are nowadays very important for the treatment of severe or incurable diseases. The manufacturing of such modalities is complex and costly, including the downstream processing, which is highly essential to ensure the safety and quality of the product. LÄS MER

  3. 2. Controlled manipulation of microparticles utilizing magnetic and dielectrophoretic forces

    Författare :LarsErik Johansson; Sven Oscarsson; Leif Nyholm; Mälardalens högskola; []
    Nyckelord :Dielectrophoresis; Magnetic beads; MEMS; Micro-particles; Micro-scale manipulation; Micro-system; Biotechnology Chemical Engineering; bioteknik kemiteknik;

    Sammanfattning : This thesis presents some experimental work in the area of manipulation of microparticles. Manipulation of both magnetic and non magnetic beads as well as microorganisms are addressed. The work on magnetic bead manipulation is focused on controlled transport and release, on a micrometer level, of proteins bound to the bead surface. LÄS MER

  4. 3. Molecular Tools for Nucleic Acid Analysis

    Författare :Deirdre O'Meara; KTH; []
    Nyckelord :nucleic acid capture; modular probes; biosensor; bio-magnetic separation; hepatitis C; sequencing; pyrosequencing; mutation detection; HIV-1; drug resistance; SNP; allele-specific extension; apyrase; genotyping.;

    Sammanfattning : Nucleic acid technology has assumed an essential role invarious areas ofin vitrodiagnostics ranging from infectious diseasediagnosis to human genetics. An important requirement of suchmolecular methods is that they achieve high sensitivity andspecificity with a fast turnaround time in a cost-effectivemanner. LÄS MER

  5. 4. Acoustofluidic preparation of whole blood components

    Författare :Anke Urbansky; NanoLund: Centre for Nanoscience; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; acoustophoresis; ultrasound; microfluidics; cell separation; blood; blood fractionation;

    Sammanfattning : Whole blood is rich in information about the physical state of an individual and is routinely used in a variety of clinical and research applications. In this thesis, the use of a microfluidic technique called acoustophoresis for separation of different blood components is explored. LÄS MER

  7. 5. Strategies for developing peptide and protein selective plastic antibodies

    Författare :Rashmi Mahajan; Ian A. Nicholls; Subramanian Suriyanarayanan; Börje Sellergren; Alessandra Maria Bossi; Linnéuniversitetet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Competitive assays; molecularly imprinted polymer; magnetic nanoparticle; nanogel; nanoparticle; plastic antibody; solid-phase synthesis; QCM.; Biokemi; Biochemistry;

    Sammanfattning : The objective of the thesis was to explore approaches for the development of molecularly imprinted materials with predetermined selectivity for peptide or protein targets. In particular, the work has focused on molecularly imprinted nanoparticles synthesized using solid-phase synthesis protocols. LÄS MER