Sökning: "Lysosomal enzyme"

Visar resultat 1 - 5 av 27 avhandlingar innehållade orden Lysosomal enzyme.

  1. 1. Engineering enzymes towards biotherapeutic applications using ancestral sequence reconstruction

    Författare :Natalie Hendrikse; Per-Olof Syrén; Johan Rockberg; Erik Nordling; Reinhard Sterner; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; enzyme engineering; enzyme evolution; ancestral sequence reconstruction; therapeutic enzymes; terpene cyclases; ammonia-lyases; sulfatases; lysosomal enzymes; Kemi; Chemistry;

    Sammanfattning : Enzymes are versatile biocatalysts that fulfill essential functions in all forms of life and, therefore, play an important role in health and disease. One specific application of enzymes in life science is their use as biopharmaceuticals, which typically benefits from high catalytic activity and stability. LÄS MER

  2. 2. Effects of Total Parenteral Nutrition on the Exocrine and Endocrine Pancreas. An Experimental Study

    Författare :Bo-Guang Fan; Lund Kirurgi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; orthopaedics; Surgery; TPN; Parotid glands; Lysosomal enzyme; Insulin; Hypotrophy; Hyperlipidemia; Food passage; Endocrine pancreas; Cholecystokinin; Exocrine pancreas; traumatology; Kirurgi; ortopedi; traumatologi; Gastro-enterology; Gastroenterologi;

    Sammanfattning : During total parenteral nutrition (TPN) adequate nutrition is provided intravenously and the enteral route is bypassed. The integrity of the gastrointestinal (GI) tract, however, is dependent on the passage of food through it. This thesis deals with the effects of TPN on the pancreas and the parotid glands. LÄS MER

  3. 3. Quality of Frozen Rainbow Trout. Effects of different freezing and thawing treatments

    Författare :Katarina Nilsson; Chalmers University of Technology; []
    Nyckelord :membrane disintegration; liquid loss; centrifuged tissue fluid; fish; lysosomal enzyme; frozen storage; glazing; freezing; sensory quality; rainbow trout; thawing;

    Sammanfattning : The effects of different freezing, frozen storage and thawing treatments on the muscle tissue of farmed rainbow trout (Oncorhychus mykiss) were studied. Biochemical measurements were correlated to sensory estimated quality changes. The freezing process affects the proteins, the lipid fractions and the membrane structures in the fish muscle. LÄS MER

  4. 4. The Lysosomal-Mitochondrial Axis Theory of Apoptosis

    Författare :Ming Zhao; Sten Orrenius; Linköpings universitet; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : In many cases, apoptosis may be initiated by a minor lysosomal destabilization, which some time later is followed by a secondary, more pronounced, lysosomal rupture. After exposure to low concentrations of sphingosine, a lysosomotropic detergent, Jurkat and J774 cells underwenr apoptotic cell death, while cells exposed to higher concentrations of this agent showed necrosis. LÄS MER

  5. 5. Cellular mechanisms involved in bone resorption

    Författare :Ulf Lerner; Umeå universitet; []
    Nyckelord :Bone resorption. cyclic AMP; parathyroid hormone; prostaglandins; la OH D3; lysosomal enzyme release; osteoclast; periodontitis; rheumatoid arthritis;

    Sammanfattning : The effects of parathyroid hormone (PTH), prostaglandins (PGE1, PGE2, PGF2a), cAMP, cAMP-analogues, phosphodiesterase (PDE) inhibitors and la (OH) D3 on bone resorption and associated cellular process have been studied in a bone organ culture system using half- calvaria from 6-7 day-old mice. Bone resorption was assessed by determining the release of calcium (Ca2+), inorganic phosphate (Pi) and 45Ca from the calvarial bones to the culture media. LÄS MER