Sökning: "Lisha"

Hittade 3 avhandlingar innehållade ordet Lisha.

  1. 1. Corneal cellular proliferation and wound healing

    Författare :Lisha Gan; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :cornea; cell proliferation; wound healing; stem-cells; leukocytes; PCNA; hyaluronan; alkali injury; photorefractive keratectomy;

    Sammanfattning : Background: Cellular proliferation plays an important role in both physiological and pathological processes. Epithelial hyperplasia in the epithelium, excessive scar formation in retrocorneal membrane formation and neovascularization are examples of excessive proliferation of cornea cells. Lack of proliferative ability causes corneal degeneration. LÄS MER

  3. 2. NMR structral studies on ruthenium complexes binding to DNA

    Författare :Lisha Wu; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NMR; DNA binding; Ru-complex dimer; CD; fluorescence; molecular dynamics simulation;

    Sammanfattning : The Ru-complex Λ,Λ-[bidppz(bipy)4Ru2]4+ (Λ,Λ-B) exhibits multiple modes of interactions with the palindromic DNA dodecamer [d(CGCGAATTCGCG)]2, however excluding any type of intercalation as shown by both optical spectroscopy and NMR. NMR observation of Λ,Λ-B titration into a DNA solution results in both a transient binding, with observation of fast exchange for protons located near the ends of the DNA duplex, and a more stable interaction in the DNA minor groove indicated by a slow exchange mechanism and binding sites for G2, G4 and A5 of the same DNA strand. LÄS MER

  4. 3. Unveiling biomacromolecule interactions-NMR and optical spectroscopy studies on ligand binding to DNA and lysozyme

    Författare :Lisha Wu; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ruthenium compounds; intercalation; Hoechst 33258; CD; fluorescence; DNA; structure rearrangements.; lysozyme; NMR;

    Sammanfattning : Studies on intercalation of bulky binuclear ruthenium compounds into DNA have attracted attention due to their slow dissociation rate and sequence selectivity. Previous results showed that dumb-bell shaped molecules of the type [μ-(bidppz)(L)4Ru2]4+, L=phenanthroline or bipyridine, bind rapidly on the surface of DNA prior to intercalation. LÄS MER