Sökning: "Large unilamellar vesicles"
Visar resultat 1 - 5 av 8 avhandlingar innehållade orden Large unilamellar vesicles.
1. Biophysical studies of peptides with functions in biotechnology and biology
Sammanfattning : My thesis concerns spectroscopic studies (NMR, CD and fluorescence) of peptides with functions in biotechnology and biology, and their interactions with a model membrane (large unilamellar phospholipid vesicles).The resorufin-based arsenical hairpin binder (ReAsH) bound to a short peptide is a useful fluorescent tag for genetic labeling of proteins in living cells. LÄS MER
2. Oligonucleotide Complexes with Cell-Penetrating Peptides : Structure, Binding, Translocation and Flux in Lipid Membranes
Sammanfattning : The ability of cell-penetrating peptides to cross plasma membranes has been explored for various applications, including the delivery of bioactive molecules to inhibit disease-causing cellular processes. The uptake mechanisms by which cell-penetrating peptides enter cells depend on the conditions, such as the cell line the concentration and the temperature. LÄS MER
3. The self-spreading double bilayer/Advances in lipid membrane nanotechnology
Sammanfattning : In my thesis I describe the generation, characterization and uses of self-spreading double bilayers. This new type of solid-supported model membrane combines features and properties of the 2D lipid bilayer membrane, and the 3D phospholiposome. The double bilayer membrane, i.e. LÄS MER
4. Interactions of the Cell-Penetrating Peptide Penetratin with Model Membranes and Live Cells
Sammanfattning : The delivery of large, hydrophilic molecules, such as proteins and oligonucleotides, to the cytoplasm and nucleus of cells is problematic due to their generally low plasma membrane permeability. With the identification of a number of cell-penetrating peptides, capable of mediating non-endocytotic, intracellular delivery of macromolecular cargos, this obstacle now seems surmountable. LÄS MER
5. Probing Proton Translocation in Influenza A/M2 Proteoliposomes - A systematic Approach to Membrane-Protein Reconstitutions
Sammanfattning : An improved method for reconstituting membrane proteins into artificial liposomes for quantitative functional analysis is presented. A number of key parameters for reconstitution by detergent removal are assessed in this thesis: The lipid-to-protein ratio, the detergent-to-lipid ratio and the lipid and cholesterol composition. LÄS MER