Sökning: "LIPASE-B"
Visar resultat 1 - 5 av 52 avhandlingar innehållade ordet LIPASE-B.
1. Rational redesign of Candida antarctica lipase B
Sammanfattning : This thesis describes the use of rational redesign to modify the properties of the enzyme Candida antarctica lipase B. Through carefully selected single-point mutations, we were able to introduce substrate-assisted catalysis and to alter the reaction specificity. LÄS MER
2. Expression and Mutagenesis studies of Candida antactica lipase B
Sammanfattning : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. LÄS MER
3. Exploring Conjugate Addition Activity in Pseudozyma antarctica Lipase B
Sammanfattning : Multifunctional enzymes have alternative functions or activities, known as “moonlighting” or “promiscuous”, which are often hidden behind a native enzyme activity and therefore only visible under special environmental conditions. In this thesis, the active-site of Pseudozyma (formerly Candida) antarctica lipase B was explored for a promiscuous conjugate addition activity. LÄS MER
4. The three-dimensional structure of Lipase B from Candida antarctica
Sammanfattning : .... LÄS MER
5. Chemo-enzymatic epoxidation: Activity and stability of Candida antarctica lipase B
Sammanfattning : Growing concerns about climate change and depleting fossil feedstocks are driving a shift within the chemical industry from energy-intensive processes to more environmentally sound and sustainable processing using renewable feedstocks. One such process is the utilization of lipases for the chemo-enzymatic epoxidation of vegetable oils and fatty acids. LÄS MER