Sökning: "Klenow fragment"

Hittade 2 avhandlingar innehållade orden Klenow fragment.

1. 1. Mathematical modelling and analysis of the pyrosequencing reaction system

   Författare :Anna Svantesson; Jeanette Hellgren Kotaleski; Anders Lansner; Pål Nyrén; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; apyrase; bioluminescence; DNA polymerase; DNA sequencing; enzyme kinetics; Klenow fragment; mathematical modelling; Michaelis-Menten; pyrosequencing; Computer science; Datalogi;

   Sammanfattning : .... LÄS MER

3. 2. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

   Författare :Jonas Eriksson; KTH; []
   Nyckelord :bioluminescence; osmolytes; glycine betaine; thermostability; firefly luciferase; inorganic pyrophosphatase; inorganic pyrophosphate; Pyrosequencing technology; secondary DNA-structures; Sequenase; Klenow-polymerase; reaction rates; temperature;

   Sammanfattning : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. LÄS MER