Sökning: "Karin Markides"

Visar resultat 6 - 10 av 18 avhandlingar innehållade orden Karin Markides.

  1. 6. Development of Enhanced Analytical Methodology for Lipid Analysis from Sampling to Detection : A Targeted Lipidomics Approach

    Författare :Giorgis Isaac; Jonas Bergquist; Karin Markides; Monica Waldebäck; William Christie; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; electrospray ionization; lipids; liquid chromatography; mass spectrometry; pesticides; phosphatidylcholine; pressurized fluid extraction; sphingomyelin; sulfatides; Analytisk kemi; Analytical chemistry; Analytisk kemi;

    Sammanfattning : This thesis covers a wide range of analytical method development for lipid analysis in complex biological samples; from sample preparation using pressurized fluid extraction (PFE) and separation with reversed phase capillary liquid chromatography (RP-LC) to detection by electrospray ionization mass spectrometry (ESI/MS) and tandem MS.The requirements for fast, reliable and selective extraction methods with minimal usage of solvents have accelerated the development of new extraction techniques. LÄS MER

  3. 7. Column Development in Capillary Electrophoresis and Electrochromatography for Bioanalytical Applications

    Författare :Nina Johannesson; Jonas Bergquist; Karin Markides; Abdul Malik; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Capillary electrophoresis CE ; Capillary electrochromatography CEC ; Mass spectrometry MS ; Bioanalysis; Sample pretreatment; Sol-gel; Analytisk kemi;

    Sammanfattning : Analysis of biological samples can be a difficult task. This thesis covers a broad aspect of the analytical areas of capillary electrophoresis (CE) and capillary electrochromatography (CEC) in combination with mass spectrometry (MS) that are of great importance for achieving fast, accurate and sensitive bioanalyses. LÄS MER

  4. 8. Liquid Chromatography – Mass Spectrometry Analysis of Short-lived Tracers in Biological Matrices : Exploration of Radiotracer Chemistry as an Analytical Tool

    Författare :Martin Lavén; Karin Markides; Bengt Långström; Rolf Ekman; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; liquid chromatography - mass spectrometry; radiotracer chemistry; positron emission tomography; biological samples; radionuclide imaging; Analytisk kemi; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Liquid chromatography – mass spectrometry (LC-MS) methods were developed for the analysis of positron emission tomography (PET) radiotracers in biological matrices. Additionally, radiotracer chemistry was explored as an analytical tool for supporting LC-MS method development and imaging molecular interactions in miniaturised chemical analysis systems. LÄS MER

  5. 9. Development of Field-adapted Analytical Methods for the Determination of New Antimalarial Drugs in Biological Fluids

    Författare :Niklas Lindegårdh; Karin Markides; Yngve Bergqvist; Anders Colmsjö; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; LC; liquid chromatography; biological samples; SPE; solid phase extraction; validation; antimalarial drugs; amodiaquine; piperaquine; atovaquone; dried blood spots; Analytisk kemi; Analytical chemistry; Analytisk kemi; Analytical Chemistry; analytisk kemi;

    Sammanfattning : This thesis deals with the development of analytical methods for the determination of new antimalarial drugs in biological fluids. The goal was to develop methods that facilitate clinical studies performed in the field, such as capillary blood sampling onto sampling paper. LÄS MER

  7. 10. Liquid Chromatography Coupled to Mass Spectrometry : Implementation of Chemometric Optimization and Selected Applications

    Författare :My Moberg; Jonas Bergquist; Karin E. Markides; Dan Bylund; Douglas B. Kell; Uppsala universitet; []
    Nyckelord :Analytical chemistry; liquid chromatography; mass spectrometry; tandem mass spectrometry; electrospray ionization; inductively coupled plasma; chemometrics; optimization; multivariate data analysis; design of experiments; response surface model; siderophore; azathioprine; peptide; Analytisk kemi;

    Sammanfattning : Liquid chromatography (LC) coupled to mass spectrometry (MS) offers highly selective and sensitive analysis of a wide variety of compounds. However, the use of hyphenated experimental set-ups implies that many parameters may have an effect on the studied response. LÄS MER