Sökning: "Isothermal DNA amplification"

Visar resultat 1 - 5 av 11 avhandlingar innehållade orden Isothermal DNA amplification.

1. 1. Visual and magnetic detection of antimicrobial resistance genes using nanoparticles and isothermal amplification

   Författare :Darío Sánchez Martín; Teresa Zardán Gómez de la Torre; Maria Strömme; Mahiar Hamedi; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA amplification; isothermal amplification; visual detection; nanoparticles; VAM-NDA; rolling circle amplification; phi29 DNA polymerase; padlock probes.; Teknisk fysik med inriktning mot nanoteknologi och funktionella material; Engineering Science with specialization in Nanotechnology and Functional Materials;

   Sammanfattning : Antibiotic resistance is becoming an increasing concern, necessitating new ways to diagnose and treat it rapidly and accurately. One proposed solution is the use of nucleic acid amplification tests, capable of detecting resistance in just a few hours, as opposed to the several days that traditional testing methods may require. LÄS MER

3. 2. Padlock Probe-Based Nucleic Acid Amplification Tests : Point-of-care Diagnostics of Infectious Diseases

   Författare :Sibel Ciftci; Mats Nilsson; Jonas Klingström; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Nucleic Acid Amplification; Isothermal Amplification Methods; Padlock Probes; Rolling Circle Amplification; Molecular Diagnostics; Infectious Disease Diagnostics; Virus; Point-of-Care; Biochemistry; biokemi;

   Sammanfattning : Recent advancements in molecular biology and biotechnology have pushed the field of molecular diagnostics much further to benefit the society towards smart access for rapid and simplified health care. In this context, point-of-care (PoC) technologies that bring the inventions in diagnostics closer to bedside settings draw attention. LÄS MER

4. 3. Novel planar and particle-based microarrays for point-of-care diagnostics

   Författare :Gustav Svedberg; Helene Svahn Andersson; Jesper Gantelius; Fridtjof Lund-Johansen; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Planar microarrays; Particle-based microarrays; Point-of-care diagnostics; Colorimetric detection; Signal enhancement; Isothermal DNA amplification; Solid-phase DNA amplification; Biotechnology; Bioteknologi;

   Sammanfattning : Point-of-care assays are easy-to-use, portable and inexpensive tests that canbe used to aid diagnostics by measuring levels of disease-specific moleculesin settings where access to advanced laboratory equipment and trainedpersonnel are limited, such as at the patient's bedside or in low resourceparts of developing countries. In order to achieve high multiplexingcapacities, such assays can be based on planar microarrays consisting ofspots immobilized on a flat surface or on particle-based microarrays basedon populations of encoded particles. LÄS MER

5. 4. Sample-to-answer paper-based nucleic acid amplification tests

   Författare :Georgios Chondrogiannis; Mahiar Hamedi; Jeff Mold; Keith Pardee; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Fiber- och polymervetenskap; Fibre and Polymer Science;

   Sammanfattning : Nucleic Acid Amplification Tests (NAATs) with PCR technology to amplify DNA, are the golden standard for infectious disease diagnostics, but they require benchtop instruments and trained users to be performed. For this reason, we all had to send PCR test to centralized laboratories during the Covid-19 pandemic. LÄS MER

7. 5. Genetic Analyses using Rolling Circle or PCR Amplified Padlock Probes

   Författare :Johan Banér; Sanjay Tyagi; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Padlock probes; rolling circle amplification; PCR; genotyping; microarray; expression analysis; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Medical Genetics; medicinsk genetik;

   Sammanfattning : Padlock probes are useful in a variety of genetic applications, some of which require that the probes are amplified in order to generate detectable signals. Two general padlock amplification methods, RCA and PCR, are discussed in this thesis. LÄS MER