Sökning: "IgG-binding domains"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden IgG-binding domains.

1. 1. Engineering of small IgG binding domains for antibody labelling and purification

   Författare :Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Bioteknologi; Biotechnology;

   Sammanfattning : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. LÄS MER

3. 2. Evolutionary and functional studies of protein H: a surface molecule of Streptococcus pyogenes

   Författare :Inga-Maria Frick; Infektionsmedicin; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; intracellular transportation; adhesion; aggregation; FNIII domain; albumin-binding; M-like protein; convergent evolution; protein G; Streptococcus pyogenes; IgG-binding; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

   Sammanfattning : Several bacterial species express surface proteins with affinity for the Fc part of human IgG. This thesis describes evolutionary and functional studies of protein H, an IgGFc-binding surface protein of the human pathogen Streptococcus pyogenes. LÄS MER

4. 3. Site-specific labeling of affinity molecules for in vitro and in vivo studies

   Författare :Anna Perols; Amelie Eriksson Karlström; Christian Hackenberger; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; molecular imaging; site-specific labeling; solid phase peptide synthesis; IgG-binding domains; photoconjugation.; Bioteknologi; Biotechnology;

   Sammanfattning : The thesis is focused on site-specific labeling of affinity molecules for different applications where two types of binding proteins, Affibody molecules and antibodies, have been used. For the purpose of improving the properties of Affibody molecules for in vivo imaging, novel bi-functional chelators for radiolabeling using the radionuclide 111In were evaluated. LÄS MER

5. 4. Combinatorial protein engineering applied to enzyme catalysis and molecular recognition

   Författare :Malin Eklund; KTH; []
   Nyckelord :phage display; selection; enzyme; affinity domains; crystal structure; affibody; lipase; protein engineering; protein A; assembly;

   Sammanfattning : The recent development of methods for constructing andhandling large collections (libraries) of proteins, from whichvariants with desired traits can be isolated, hasrevolutionized the field of protein engineering. Key elementsof such methods are the various ways in which the genotypes(the genes) and the phenotypes (the encoded proteins) arephysically linked during the process. LÄS MER

7. 5. Molecular analysis of Streptococcus pyogenes and its interactions with the human host

   Författare :Andreas Berge; Institutionen för experimentell medicinsk vetenskap; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; complement activation; fibrinogen; IgG; M protein; C5a peptidase; streptococcal cysteine proteinase; streptokinase; t-PA; Streptococcus pyogenes; plasminogen; C1q; insertional sequence.; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

   Sammanfattning : Streptococcus pyogenes causes infections only in humans, from trivial tonsillitis to life-threatening conditions. One of its most important virulence factors is the M-like protein family conferring resistance to phagocytosis to the bacterium. The M-like proteins are surface exposed rod-like molecules attached to the cell wall. Each strain of S. LÄS MER