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Visar resultat 1 - 5 av 7 avhandlingar som matchar ovanstående sökkriterier.

  1. 1. The Membrane-Spanning Domain of Complex I Investigated with Fusion Protein Techniques

    Författare :Maria Trane; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NuoN; NuoM; NuoL; covalent bound heme; Bacillus subtilis; Escherichia coli; membrane protein; His-tag; Cytochrome c; NADH:quinone oxidureductase; fusion protein;

    Sammanfattning : NADH:quinone oxidoreductase, or complex I, is a large, complex and poorly understood bioenergetic enzyme in the respiratory chain of living organisms. The enzyme has a conserved core structure, comprising fourteen protein subunits: Seven subunits protrude from the membrane and contain a flavin prosthetic group and eight iron-sulfur clusters that guide electrons from the oxidation of NADH towards the quinone binding site(s). LÄS MER

  3. 2. Ribosomal Stalk Protein L12 : Structure, Function and Application

    Författare :Chandra Sekhar Mandava; Suparna Sanyal; Knud Nierhaus; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribosome; protein synthesis; L12 dimer; initiation; elongation; G factors; and His-tag; Molecular biology; Molekylärbiologi; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Sammanfattning : Ribosomal stalk proteins are known to play important role in protein synthesis. The ‘stalk’, an extended structure on the large subunit of the ribosome is composed mainly of two to three dimers of L12 and one L10 protein, which forms the base of the stalk. In E. LÄS MER

  4. 3. Protein production, characterization and structure determination in structural genomics

    Författare :Esmeralda A. Woestenenk; Torleif Härd; Göran Karlsson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; Biology; Biologi;

    Sammanfattning : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. LÄS MER

  5. 4. Structural Studies of the Human Water Channel Protein Aquaporin 5

    Författare :Anna Backmark; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; aquaporin 5; aquaporins; X-ray crystallography; twinning; structure; trafficking;

    Sammanfattning : Aquaporins are water channel proteins found in the membranes of all living cells. In humans, thirteen aquaporins have been identified to date. Many of them have been implicated in water imbalance disorders. Water transport through the aquaporins is regulated at several levels. LÄS MER

  7. 5. Glycoside hydrolases from extremophiles isolated in Iceland. alpha-L-Rhamnosidases and pectin degrading enzymes

    Författare :Hakon Birgisson; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immobilization; Biotechnology; Bioteknik; crystallization; sequencing; cloning; screening; thermophile; psychrotroph; psychrophile; alpha-L-arabinofuranosidase; alpha-L-rhamnosidase; thermostable; cold active; polygalacturonase;

    Sammanfattning : Extreme environments (high and low temperature, high and low pH, etc.) are inhabited by a vast and diverse flora of microorganisms, which are adapted to these environments (extremophiles). LÄS MER