Sökning: "High-Throughput Sequencing"

Visar resultat 1 - 5 av 163 avhandlingar innehållade orden High-Throughput Sequencing.

  1. 1. Library Preparation for High Throughput DNA Sequencing

    Författare :Anders Jemt; Joakim Lundeberg; Petri Auvinen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; sequencing; massively parallel sequencing; library preparation; automation; genome; transcriptome; Bioteknologi; Biotechnology;

    Sammanfattning : Order 3 billion base pairs of DNA in the correct order and you get the blueprint of a human, the genome. Before the introduction of massively parallel sequencing a little more than a decade ago it would cost around $10 million to get this blueprint. LÄS MER

  3. 2. Droplet microfluidics for high throughput biological analysis

    Författare :Håkan Jönsson; Helene Andersson-Svahn; Andrew Griffiths; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microfluidics; Droplet microfluidics; High throughput biology; Single cell analysis; Hydrodynamic separation; Enzyme amplification; Fluorescence polarization; Microfabrication; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : Many areas of biological research increasingly perform large-scale analyses.  In genomics the entire gene repertoire of an organism is analyzed.  Proteomics attempts to understand the function and expression patterns of all proteins in a cell or organism. LÄS MER

  4. 3. High-throughput DNA Sequencingin Microbial Ecology : Methods and Applications

    Författare :Luisa Hugerth; Anders Andersson; Jeroen Raes; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microbial ecology; Baltic Sea; Next-generation sequencing; Amplicon sequencing; Metagenomics; Bioteknologi; Biotechnology;

    Sammanfattning : Microorganisms play central roles in planet Earth’s geochemical cycles, in food production, and in health and disease of humans and livestock. In spite of this, most microbial life forms remain unknown and unnamed, their ecological importance and potential technological applications beyond the realm of speculation. LÄS MER

  5. 4. Towards comprehensive cellular atlases : High-throughput cell mapping by in situ sequencing

    Författare :Xiaoyan Qian; Mats Nilsson; Gunnar von Heijne; Anthony Zador; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Spatially resolved transcriptomics; in situ sequencing; cell type; spatial analysis; Biochemistry; biokemi;

    Sammanfattning : With recent technological advancements in single-cell biology, many aspects of individual cells are characterized with unprecedented resolution and details. Cell types in human and model organisms are redefined, and multiple organ-wide atlases are proposed to integrate different types of data to provide a comprehensive view of biological systems at cellular resolution. LÄS MER

  7. 5. microRNAs: from biogenesis to organismal tracing

    Författare :Wenjing Kang; Marc Friedländer; Jens Lagergren; Ulf Ørom; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; microRNA; microRNA biogenesis; taxonomical identification; high throughput sequencing; xenomiRs; RNA structure; high throughput screening; molekylär biovetenskap; Molecular Bioscience;

    Sammanfattning : MicroRNAs (miRNAs) are short noncoding RNAs of around 22 nucleotides in length, which help to shape the expression of most mRNAs. Perturbation of miRNA expression has revealed a variety of defects in development, cell specification, physiology and behavior. LÄS MER