Sökning: "High throughput sequencing"

Visar resultat 21 - 25 av 170 avhandlingar innehållade orden High throughput sequencing.

  1. 21. High-throughput protein analysis using mass spectrometry-based methods

    Författare :Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
    Nyckelord :Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

    Sammanfattning : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. LÄS MER

  3. 22. Improved statistical methodology for high-throughput omics data analysis

    Författare :Wenjiang Deng; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Over the last two decades, the advent of high-throughput omics technology has substantially revolutionized biological and biomedical research. A large volume of omics data has been produced with the rapid development of sequencing techniques. LÄS MER

  4. 23. Perturbance and Stimulation : using Nitrogen Addition and High-Throughput Sequencing to Study Fungal Communities in Boreal Forests

    Författare :Andreas N. Schneider; Nathaniel Street; Vaughan Hurry; Torgny Näsholm; Colin Averill; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Boreal forest; Scots pine; Norway spruce; ectomycorrhiza; saprotroph; fertilization; nitrogen; mycobiome; rhizosphere; decomposition; carbon; seedling establishment; forest management; clearcutting;

    Sammanfattning : Fungal communities are major players in globally important nutrient cycling processes, and form symbioses with most terrestrial plants. In the nitrogen (N) limited Swedish boreal forest, ectomycorrhizal (EcM) fungi colonize most roots of the economically important and stand dominating conifer species, Norway spruce (Picea abies) and Scots pine (Pinus sylvestris), with significant implications for tree nutrition and decomposition processes. LÄS MER

  5. 24. Genome-Wide Studies of Transcriptional Regulation in Human Liver Cells by High-throughput Sequencing

    Författare :Madhusudhan Reddy Bysani; Claes Wadelius; Mattias Mannervik; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ChIP-seq; Transcription factors; Alcoholic steatohepatitis; Genome-wide; GWAS; SNPs;

    Sammanfattning : The human genome contains slightly more than 20 000 genes that are expressed in a tissue specific manner. Transcription factors play a key role in gene regulation. By mapping the transcription factor binding sites genome-wide we can understand their role in different biological processes. LÄS MER

  7. 25. Software Tools and Algorithms for Shotgun Sequence Assembly

    Författare :Martti T. Tammi; Bruce Roe; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Shotgun sequencing; multiple alignment; fragment assembly; repeats; sequencing error; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : During the last ten years, a genomics revolution has changed the ways biological research is carried out. The draft sequence of the human genome is available, as well as the sequence of 84 other completed genomes. LÄS MER