Sökning: "High throughput sequencing"

Visar resultat 11 - 15 av 170 avhandlingar innehållade orden High throughput sequencing.

  1. 11. Methods to Prepare DNA for Efficient Massive Sequencing

    Författare :Sverker Lundin; Joakim Lundeberg; Harold Swerdlow; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA; Massive sequencing; Next Generation Sequencing; Library Preparation; Barcoding; Multiplexing;

    Sammanfattning : Massive sequencing has transformed the field of genome biology due to the continuous introduction and evolution of new methods. In recent years, the technologies available to read through genomes have undergone an unprecedented rate of development in terms of cost-reduction. LÄS MER

  2. 12. Improved Techniques for High-Throughput Molecular Diagnostics

    Författare :Mario Curcio; KTH; []
    Nyckelord :DNA; Molecular Diagnostics;

    Sammanfattning : The amount of information derived from sequencing the humangenome is leading to an exponential increase in the rate atwhich genes and genetic disorders are mapped and characterized.As a consequence, the demand for genetic testing is alsodramatically increasing. LÄS MER

  3. 13. Culture-independent methods and high-throughput sequencing applied to evolutionary microbial genomics

    Författare :Anders E. Lind; Thijs JG Ettema; Purificación López-García; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Single cell genomics; metagenomics; amplicon; archaea; haloarchaea; endosymbiosis; ciliate; hydrogenosome; tree reconciliation;

    Sammanfattning : Culture-independent methods allow us to better understand the diversity of microorganisms on earth. By omitting the culturing step, we can gain access to species previously out of our reach.To better capture and understand the diversity of microorganisms, we have developed an alternative to 16S amplicon sequencing. LÄS MER

  4. 14. Strategies for de novo DNA sequencing

    Författare :Anna Blomstergren; KTH; []
    Nyckelord :DNA sequencing; DNA purification; automation; solid-phase; streptavidin; biotin; modular probes; Helicobacter pylori; cag PAI;

    Sammanfattning : The development of improved sequencing technologies hasenabled the field of genomics to evolve. Handling andsequencing of large numbers of samples require an increasedlevel of automation in order to obtain high throughput andconsistent quality. LÄS MER

  5. 15. Enabling massive genomic and transcriptomic analysis

    Författare :Henrik Stranneheim; Joakim Lundeberg; Elaine Mardis; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; sequencing; massively parallel sequencing; alignment; assembly; single nucleotide polymorphism; LHON;

    Sammanfattning : In recent years there have been tremendous advances in our ability to rapidly and cost-effectively sequence DNA. This has revolutionized the fields of genetics and biology, leading to a deeper understanding of the molecular events in life processes. LÄS MER