Sökning: "High throughput biology"

Visar resultat 11 - 15 av 204 avhandlingar innehållade orden High throughput biology.

  1. 11. Bioinformatic tools for the analysis of microbial genomes

    Författare :Thomas Sicheritz-Pontén; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Developmental biology; Utvecklingsbiologi; Developmental biology; Utvecklingsbiologi; molekylärbiologi; Molecular Biology;

    Sammanfattning : High throughput genome sequencing projects are producing an enormous amount of rawdata which has to be converted into biological knowledge. The newly established field ofbioinformatics is concerned with the continuous development of novel methods and toolsfor the comparative analysis of DNA sequences and genomic data. LÄS MER

  3. 12. Robustness quantification in yeast: A methodology to study phenotypic, evolutionary, and genomic aspects of microbial robustness

    Författare :Cecilia Trivellin; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; bioproduction; Saccharomyces cerevisiae; adaptive laboratory evolution; High-throughput; fluctuating conditions; perturbations;

    Sammanfattning : Bioprocesses contribute to the shift towards a more sustainable economy. In bioprocesses, valuable chemicals can be generated from renewable resources while, at the same time, reducing carbon emissions. A major hurdle in bringing bio-based products to market is the time and cost involved in designing efficient cell factories. LÄS MER

  4. 13. Methodology for high-throughput production of soluble recombinant proteins in Escherichia coli

    Författare :Katrin Markland; Gen Larsson; Pär Nordlund; Jenny Ottosson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; high-throughput methodology; recombinant protein production; solubility; productivity; quality; cultivation technology; parallel reactors; Biochemistry; Biokemi;

    Sammanfattning : The aim of this work was to investigate and determine central parameters that can be used to control and increase the solubility, quality and productivity of recombinant proteins. These central parameters should be applicable under the constraints of high-throughput protein production in Escherichia coli. LÄS MER

  5. 14. Microwell devices for single-cell analyses

    Författare :Sara Lindström; Helene Andersson-Svahn; Mehmet Toner; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; microwell; miniaturization; microfluidics; cell culture; single-cell; clone; imaging; stem cell; cancer; low volume; high-throughput; Molecular biology; Molekylärbiologi;

    Sammanfattning : Powerful tools for detailed cellular studies are emerging, increasing the knowledge ofthe ultimate target of all drugs: the living cell. Today, cells are commonly analyzed inensembles, i.e. thousands of cells per sample, yielding results on the average responseof the cells. LÄS MER

  7. 15. Culture-independent methods and high-throughput sequencing applied to evolutionary microbial genomics

    Författare :Anders E. Lind; Thijs JG Ettema; Purificación López-García; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Single cell genomics; metagenomics; amplicon; archaea; haloarchaea; endosymbiosis; ciliate; hydrogenosome; tree reconciliation;

    Sammanfattning : Culture-independent methods allow us to better understand the diversity of microorganisms on earth. By omitting the culturing step, we can gain access to species previously out of our reach.To better capture and understand the diversity of microorganisms, we have developed an alternative to 16S amplicon sequencing. LÄS MER