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Visar resultat 1 - 5 av 41 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Development of molecular recognition by rational and combinatorial engineering

   Författare :Andreas Jonsson; Stefan Ståhl; Mats Persson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecule; albumin binding domain; protein engineering; phage display; amyloid beta peptide; TNF; HSA; Molecular biology; Molekylärbiologi;

   Sammanfattning : Combinatorial protein engineering, taking advantage of large libraries of protein variants and powerful selection technology, is a useful strategy for developing affinity proteins for applications in biotechnology and medicine. In this thesis, two small affinity proteins have been subjected to combinatorial protein engineering to improve or redirect the binding. LÄS MER

3. 2. Epigenetic dysregulation in relation to psychiatric traits in adolescence and adulthood

   Författare :Adrian Desai Boström; Helgi B. Schiöth; Daniel Nätt; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; methylome-wide; DNA methylation; hypersexual disorder; microRNA; oxytocin signaling; psychiatry; hsa-miR-4456; microRNA-4456; MIR4456; epigenetic dysregulation; microRNA expression; gene target prediction; epigenetics; suicide; suicide attempt; HPA axis; CRH gene; Epigenetics; Methylation; Adolescent depression; MIR4646; methylome-wide association study; Omega-3; mQTL analysis; GAD1; HDAC3; DAWBA;

   Sammanfattning : Epigenetics has evolved into a key research focus in the field of psychiatry. DNA methylation is the most researched epigenetic mechanism. In paper I-III, 130 and 93 adolescents were randomly recruited at two separate intervals. LÄS MER

4. 3. Protein engineering to explore and improve affinity ligands

   Författare :Martin Linhult; KTH; []
   Nyckelord :binding; affinity; human serum albumin HSA ; albumin-binding domain ABD ; affinity chromatography; deamidation; protein A; stabilization; Z-domain; capacity; protein G; cleaning-in-place CIP ; protein engineering; C2 receptor;

   Sammanfattning : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. LÄS MER

5. 4. Electrospray Ionization Mass Spectrometry for Determination of Noncovalent Interactions in Drug Discovery

   Författare :Kurt Benkestock; Johan Roeraade; Renato Zenobi; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; electrospray ionization; drug discovery; noncovalent interaction; complexes; human serum albumin HSA ; fatty acid binding protein FABP ; ribonuclease; ligand screening; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Noncovalent interactions are involved in many biological processes in which biomolecules bind specifically and reversibly to a partner. Often, proteins do not have a biological activity without the presence of a partner, a ligand. LÄS MER

7. 5. Protein adducts of hexahydrophthalic anhydride- chemical structures and biomarkers

   Författare :Monica Kåredal; Avdelningen för arbets- och miljömedicin; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; arbetsmiljömedicin; Yrkesmedicin; industrial medicine; Occupational health; nasal lavage; mass spectrometry; biomarker; human serum albumin; hemoglobin; Hexahydrophthalic anhydride; adducts;

   Sammanfattning : Organic acid anhydrides (OAAs) are low molecular weight chemicals used in the industry for manufacturing of plastics such as epoxy resins. Exposure to these chemicals may lead to symptoms from the eyes and the respiratory tract in form of conjunctivitis, rhinitis and asthma. LÄS MER