Sökning: "HSA"

Visar resultat 1 - 5 av 36 avhandlingar innehållade ordet HSA.

  1. 1. Development of molecular recognition by rational and combinatorial engineering

    Författare :Andreas Jonsson; Stefan Ståhl; Mats Persson; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Affibody molecule; albumin binding domain; protein engineering; phage display; amyloid beta peptide; TNF; HSA; Molecular biology; Molekylärbiologi;

    Sammanfattning : Combinatorial protein engineering, taking advantage of large libraries of protein variants and powerful selection technology, is a useful strategy for developing affinity proteins for applications in biotechnology and medicine. In this thesis, two small affinity proteins have been subjected to combinatorial protein engineering to improve or redirect the binding. LÄS MER

  2. 2. Protein engineering to explore and improve affinity ligands

    Författare :Martin Linhult; KTH; []
    Nyckelord :binding; affinity; human serum albumin HSA ; albumin-binding domain ABD ; affinity chromatography; deamidation; protein A; stabilization; Z-domain; capacity; protein G; cleaning-in-place CIP ; protein engineering; C2 receptor;

    Sammanfattning : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. LÄS MER

  3. 3. Electrospray Ionization Mass Spectrometry for Determination of Noncovalent Interactions in Drug Discovery

    Författare :Kurt Benkestock; Johan Roeraade; Renato Zenobi; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; electrospray ionization; drug discovery; noncovalent interaction; complexes; human serum albumin HSA ; fatty acid binding protein FABP ; ribonuclease; ligand screening; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Noncovalent interactions are involved in many biological processes in which biomolecules bind specifically and reversibly to a partner. Often, proteins do not have a biological activity without the presence of a partner, a ligand. LÄS MER

  4. 4. Functional, structural and evolutionary studies on a family of bacterial surface proteins

    Författare :Maarten de Château; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; virologi; bakteriologi; Mikrobiologi; Peptostreptococcus magnus protein L Ig light chains protein PAB albumin-binding GA module bacterial genetics module shuffling multidomain proteins protein evolution virulence; mycology; virology; bacteriology; Microbiology; mykologi;

    Sammanfattning : This thesis is concerned with a family of bacterial surface proteins expressed by different strains of the anaerobic Gram-positive, commensal and human pathogen Peptostreptococcus magnus. These proteins have been studied with respect to their role in virulence, function, structure, and evolution. LÄS MER

  5. 5. Protein adducts of hexahydrophthalic anhydride- chemical structures and biomarkers

    Författare :Monica Kåredal; Avdelningen för arbets- och miljömedicin; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; arbetsmiljömedicin; Yrkesmedicin; industrial medicine; Occupational health; nasal lavage; mass spectrometry; biomarker; human serum albumin; hemoglobin; Hexahydrophthalic anhydride; adducts;

    Sammanfattning : Organic acid anhydrides (OAAs) are low molecular weight chemicals used in the industry for manufacturing of plastics such as epoxy resins. Exposure to these chemicals may lead to symptoms from the eyes and the respiratory tract in form of conjunctivitis, rhinitis and asthma. LÄS MER