Sökning: "Green Fluorescent protein"

Visar resultat 1 - 5 av 92 avhandlingar innehållade orden Green Fluorescent protein.

1. 1. Hydrophobic Interactions in Bioseparation - Applications of Modified Green Fluorescent Protein

   Författare :Kristian Becker; Tillämpad biokemi; []
   Nyckelord :Hydrophobic interaction chromatography; capillary electrochromatography; E. coli; isolation tags; haemoglobin; green fluorescent protein; retention prediction; genetic engineering; accessible surface area;

   Sammanfattning : This thesis describes interactions that are salt-promoted and mainly based on the hydrophobicity of proteins. A series of variants of the green fluorescent protein (GFP) was first constructed by site-directed mutagenesis. Several tags with various properties were then genetically fused to the N-terminus of GFP. LÄS MER

3. 2. On bacterial formats in protein library technology

   Författare :Per-Åke Löfdahl; Per-Åke Nygren; Mikael Widersten; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody molecule; β-lactamase; combinatorial library; green fluorescent protein GFP ; protein engineering; protein fragment complementation assay PCA ; selection; TEVprotease; TNF-α; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik;

   Sammanfattning : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. LÄS MER

4. 3. Amyloid Beta – Biotherapy Target and Biotechnological Tool

   Författare :Sebastian W. Meister; Stefan Ståhl; John Löfblom; Maarten Merkx; KTH; []
   Nyckelord :Alzheimer’s disease; amyloid beta; blood-brain barrier; green-fluorescent protein; intracellular assays; proteases; protease substrate specificity; protein aggregation; protein engineering.; Bioteknologi; Biotechnology;

   Sammanfattning : Alzheimer’s disease is a progressive neurodegenerative disease characterized primarily by memory impairment and cognitive decline. As of 2020, an estimated 50 million people suffer from Alzheimer’s disease or related dementia and no disease-modifying treatment options are yet approved for clinical practice. LÄS MER

5. 4. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

   Författare :Florent Bernaudat; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

   Sammanfattning : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). LÄS MER

7. 5. Systems Biology of Protein Secretion in Human Cells: Multi-omics Analysis and Modeling of the Protein Secretion Process in Human Cells and its Application

   Författare :Rasool Saghaleyni; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Erythropoietin; cancer protein secretory pathway; protein secretion modeling; HEK293; protein secretion; Integrative omics analysis; genome-scale modeling;

   Sammanfattning : Since the emergence of modern biotechnology, the production of recombinant pharmaceutical proteins has been an expanding field with high demand from industry. Pharmaceutical proteins have constituted the majority of top-selling drugs in the pharma industry during recent years. LÄS MER