Sökning: "Genetic fluorescence label"
Hittade 4 avhandlingar innehållade orden Genetic fluorescence label.
1. Biophysical studies of peptides with functions in biotechnology and biology
Sammanfattning : My thesis concerns spectroscopic studies (NMR, CD and fluorescence) of peptides with functions in biotechnology and biology, and their interactions with a model membrane (large unilamellar phospholipid vesicles).The resorufin-based arsenical hairpin binder (ReAsH) bound to a short peptide is a useful fluorescent tag for genetic labeling of proteins in living cells. LÄS MER
2. Development of Techniques for Characterization, Detection and Protein Profiling of Extracellular Vesicles
Sammanfattning : Nanosized extracellular vesicles (EVs, ∼30-2000 nm) have emerged as important mediators of intercellular communication, offering opportunities for both diagnostics and therapeutics. In particular, small EVs generated from the endolysosomal pathway (∼30-150 nm), referred to as exosomes, have attracted interest as a suitable biomarker for cancer diagnostics and treatment monitoring based on minimally invasive liquid biopsies. LÄS MER
3. Characterizing the pancreatic "isletome" : 3D optical imaging to study diabetes
Sammanfattning : The pancreas is a specialised multipurpose organ, that can be separated into two major compartments: endocrine and exocrine. The exocrine part makes up the majority of the organ volume and functions to secrete digestive enzymes into the small intestine. LÄS MER
4. Methods for 2D and 3D Quantitative Microscopy of Biological Samples
Sammanfattning : New microscopy techniques are continuously developed, resulting in more rapid acquisition of large amounts of data. Manual analysis of such data is extremely time-consuming and many features are difficult to quantify without the aid of a computer. LÄS MER