Avancerad sökning

Visar resultat 1 - 5 av 308 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Treatment of Skin Wounds with Cell Transplantation and Gene Transfer

   Författare :Tor Svensjö; Lunds universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; pigs; gene regulation; cell culture; fibroblasts; keratinocytes; wound healing; skin; gene transfer; Transplantation; gene therapy; Surgery; orthopaedics; traumatology; Kirurgi; ortopedi; traumatologi;

   Sammanfattning : Background: A large number of different wound coverings have been used in order to improve the wound microenvironment, and thus accelerate repair. Since almost two centuries, clinicians and researchers have developed techniques for skin transplantation. LÄS MER

3. 2. Retroviral gene transfer to repopulating hematopoietic cells

   Författare :Thomas Relander; Tumörmikromiljö; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; GLVR-1; VSV-G; amphotropic; GALV; long-term bone marrow culture; NOD SCID; retroviral vector; hematopoietic stem cells; stem cells; gene transfer; gene therapy; fibronectin; Clinical genetics; Klinisk genetik;

   Sammanfattning : Hematopoietic stem cells (HSCs) have the capacity to maintain hematopoiesis throughout life. HSCs are ideal targets for permanent gene transfer, as the transgene will be expressed in all the progeny of the gene-modified stem cells. LÄS MER

4. 3. Evolutionary Dynamics of Mutation and Gene Transfer in Bacteria

   Författare :Peter A Lind; Dan I Andersson; J. Arjan G. M. de Visser; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fitness cost; bacterial evolution; gene amplification; mutational biases; GC content; synonymous substitutions; horizontal gene transfer; experimental evolution; Bacteriology; Bakteriologi; Microbiology; Mikrobiologi; Genetics; Genetik; Biology; Biologi; Mikrobiologi; Microbiology;

   Sammanfattning : The study of bacterial evolution is fundamental for addressing current problems of antibiotic resistance and emerging infectious diseases and lays a solid foundation for successful and rational design in biotechnology and synthetic biology. The main aim of this thesis is to test evolutionary hypotheses, largely based on theoretical considerations and sequence analysis, by designing scenarios in a laboratory setting to obtain experimental data. LÄS MER

5. 4. On the binding of growth-promoting polyamines to proteoglycans: Implications for growth-regulation and polycation-mediated gene transfer

   Författare :Mattias Belting; Institutionen för experimentell medicinsk vetenskap; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; cationic lipid.; gene transfer; gene therapy; cell growth; polyamine transport; spermine; polyamine; growth-inhibition; heparan sulphate; dermatan sulphate; Proteoglycan; glycosaminoglycan; cytogenetics; Genetik; cytogenetik;

   Sammanfattning : Initial investigations were directed at studying the interaction between polyamines and various glycosaminoglycans (GAGs). The polyamine spermine displayed binding to dermatan sulphate (DS) and heparan sulphate (HS) with similar (Kd, 3.9 x 10-4 M) and higher (Kd, 0.37 x 10-6 M) affinity, respectively, than to DNA. LÄS MER

7. 5. Making Doable Problems within Controversial Science : U.S. and Swedish Scientists’ Experience of Gene Transfer Research

   Författare :Hannah Grankvist; Boel Berner; Claes-Fredrik Helgesson; Margareta Hallberg; Linköpings universitet; []
   Nyckelord :Gene transfer; biomedicine; controversy; situatedness; doability; doable problems; alignment; articulation work; boundary-work; frame of reference; U.S.A.; Sweden; interviews; Genterapi; biomedicin; kontrovers; doability; utförbara problem; alignment; situatedness; articulation work; gränsarbete; referensram; USA; Sverige; intervjuer.; SOCIAL SCIENCES; SAMHÄLLSVETENSKAP;

   Sammanfattning : This thesis explores how scientists within the controversial scientific field of gene  transfer make their research doable. Based on in-depth interviews with gene transfer scientists and key individuals from different regulatory agencies and advisory boards in Sweden and the U.S.A. LÄS MER