Sökning: "GFP"

Visar resultat 21 - 25 av 167 avhandlingar innehållade ordet GFP.

1. 21. Methods for cell line and protein engineering

   Författare :Magnus Lundqvist; Johan Rockberg; Mathias Uhlén; Diethard Mattanovich; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell line development; therapeutic proteins; protein engineering; molecular cloning; mutagenesis; split-GFP; Bioteknologi; Biotechnology;

   Sammanfattning : Therapeutic proteins are becoming increasingly important. They are desirable, as they typically possess low adverse effects and higher specificity compared to the traditional, small molecule drugs. But they are also more complex and involve different intricate and expensive development and production processes. LÄS MER

3. 22. Perplexing Protein Puzzles

   Författare :Stina Lindman; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; calbindin D9k; split-GFP; protein G B1; pKa-values; protein reconstitution; Electrostatic interactions; protein stability; protein denaturation; heteronuclear NMR; pH titrations;

   Sammanfattning : Protein structure and stability are inherent in the amino acid sequence and governed by non-covalent interactions. The cooperation between forces is, however, perplexing and not well understood. In order to elucidate and predict protein folding and stability, detailed studies of non-covalent interactions are required. LÄS MER

4. 23. Unravelling protein stability and aggregation

   Författare :Tanja Weiffert; NanoLund: Centre for Nanoscience; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein stability; protein aggregation; amyloid beta peptide; protein stabilization; split GFP; monellin; secretagogin; ; aggregation kinetics; toxicity; aggregation mechanism; coaggregation;

   Sammanfattning : Proteins are complex macromolecules that are fundamental to all living species. The stability of proteins are governed by non-covalent forces within the protein and between the protein and its surrounding environment. LÄS MER

5. 24. Used but not Sensed - The Paradox of D-xylose Metabolism in Saccharomyces cerevisiae

   Författare :Karen Ofuji Osiro; Teknisk mikrobiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; saccharomyces cerevisiae; sugar sensing signalling; D-Xylose; XR XDH; GFP biosensor; cAMP PKA; Snf3p Rgt2p; SNF1 Mig1p; flow cytometry;

   Sammanfattning : The realization that the extraction and combustion of fossil fuels is having serious effects on the environment and the climate, together with the ever-growing need for fuels, has led to the development of the concept of the biorefinery. Biorefineries are refineries in which fossil resources, such as oil, are replaced by renewable biomaterials to produce biofuels and biochemicals. LÄS MER

7. 25. Staphylococcus aureus toxins - Influence on food safety and animal health

   Författare :Yusak Budi Susilo; Teknisk mikrobiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Staphylococcus aureus; toxins; food safety; enterotoxin A SEA ; prophage life cycle; GFP reporter strain; flow cytometry; enterotoxin D SED ; accessory gene regulator agr ; bovine mastitis; enterotoxin C SEC ; toxic shock syndrome toxin-1 TSST-1 ;

   Sammanfattning : Staphylococcus aureus (S. aureus) is a notorious opportunistic foodborne pathogen and also a common cause of bovine mastitis. It is known to produce many different virulence factors, including various staphylococcal enterotoxins and toxic shock syndrome toxin-1 (TSST-1). LÄS MER