Sökning: "GFP biosensor"

Hittade 5 avhandlingar innehållade orden GFP biosensor.

  1. 1. In search of a biosensor for DNT detection : Studies of inducer response and specificity of DntR

    Författare :Rosa Lönneborg; Peter Brzezinski; Ray Dixon; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DntR; transcriptional regulation; gfp; nitro-aromatic compounds; LysR family; LTTR; TNT; DNT; FACS; directed evolution; random mutagenesis; recombination; structure determination; biokemi; Biochemistry;

    Sammanfattning : The primary aim of the work presented in this thesis was to change the inducer specificity of the DntR protein in order to improve the response to DNT. The long-term goal is to use this protein in a biosensor for DNT, a signature compound for detection of the explosive TNT. LÄS MER

  3. 2. Used but not Sensed - The Paradox of D-xylose Metabolism in Saccharomyces cerevisiae

    Författare :Karen Ofuji Osiro; Teknisk mikrobiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; saccharomyces cerevisiae; sugar sensing signalling; D-Xylose; XR XDH; GFP biosensor; cAMP PKA; Snf3p Rgt2p; SNF1 Mig1p; flow cytometry;

    Sammanfattning : The realization that the extraction and combustion of fossil fuels is having serious effects on the environment and the climate, together with the ever-growing need for fuels, has led to the development of the concept of the biorefinery. Biorefineries are refineries in which fossil resources, such as oil, are replaced by renewable biomaterials to produce biofuels and biochemicals. LÄS MER

  4. 3. An NADH-Coupled Biosensor for Engineering Redox Metabolism in Saccharomyces cerevisiae

    Författare :Jan Knudsen; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Yeast; redox balance; glycerol whole-cell biocatalysis; single-cell monitoring; FACS;

    Sammanfattning : Baker’s yeast, Saccharomyces cerevisiae’s potential in industrial biotechnology for producing valuable products e.g. biofuels, bulk chemicals, bio-flavours and pharmaceuticals is established. LÄS MER

  5. 4. Hydrophobic Interactions in Bioseparation - Applications of Modified Green Fluorescent Protein

    Författare :Kristian Becker; Tillämpad biokemi; []
    Nyckelord :Hydrophobic interaction chromatography; capillary electrochromatography; E. coli; isolation tags; haemoglobin; green fluorescent protein; retention prediction; genetic engineering; accessible surface area;

    Sammanfattning : This thesis describes interactions that are salt-promoted and mainly based on the hydrophobicity of proteins. A series of variants of the green fluorescent protein (GFP) was first constructed by site-directed mutagenesis. Several tags with various properties were then genetically fused to the N-terminus of GFP. LÄS MER

  7. 5. Pancreatic beta-cell insulin signaling in genetic and dietary models of obesity and insulin resistance

    Författare :Meike Paschen; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Type 2 Diabetes Mellitus (T2DM) is a heterogeneous metabolic disease characterized by elevated blood glucose levels that has reached pandemic proportions. Genome-wide association studies have linked T2DM to the function of the insulin-producing pancreatic βcell residing in the micro-organ islet of Langerhans. LÄS MER