Sökning: "Fura-2"

Visar resultat 1 - 5 av 22 avhandlingar innehållade ordet Fura-2.

  1. 1. Motility of human polymorphonuclear leukocytes : An image processing approach

    Författare :Mikael Gustafsson; Erik Gylfe; Linköpings universitet; []
    Nyckelord :Human polymorphonuclear leukocytes; locomotion; phagocytosis; Saccharomyces cerevisiae; membrane transport; intracellular free calcium; cellular pH; water influx; actin dynamics; digital video microscopy; image processing; fura-2; BCECF; DII; MEDICINE; MEDICIN;

    Sammanfattning : Cell motility is essential for polymorphonuclear leukocyte (PMN) function in the defense against invading microorganisms and in inflammatory processes. Using digital video microscopy and image processing, individual cells were studied during random locomotion, chemotactic locomotion and phagocytosis. LÄS MER

  3. 2. Ethanol-mediated effects on astroglial cells in primary culture. With a focus on acute changes in intracellular Ca2+, cell volume, actin filaments, and gap junction coupling

    Författare :Louise Adermark; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; astrocyte; BaCl2; cell swelling; fura-2; furosemide; ouabain; scrape loading; volume estimation;

    Sammanfattning : The astroglial cells are abundant in the central nervous system (CNS), where they control and regulate the homeostasis of the extracellular milieu. A change in astroglial cell volume, mediated through activation of neuroactive substances or changes in osmolarity, leads to changes in the size and geometry of the extracellular space volume, which could affect the diffusion and concentration of neuroactive substances. LÄS MER

  4. 3. Oscillatory Ca2+ signaling in glucose-stimulated murine pancreatic β-cells : Modulation by amino acids, glucagon, caffeine and ryanodine

    Författare :Meftun Ahmed; Per Lindström; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell biology; pancreatic β-cells; islets of Langerhans; glucose; Ca2 oscillations; Ca2 transients; potassium; ryanodine; caffeine; glucagon; cAMP; inositol 1; 4; 5-trisphosphate; fura-2; glycine; alanine; arginine; Ca2 channels; rats; lean mice; ob ob mice; leptin; Cellbiologi; Cell biology; Cellbiologi; medicinsk cellbiologi; Medical Cell Biology;

    Sammanfattning : Oscillations in cytoplasmic Ca2+ concentration ([Ca2+]i) is the key signal in glucose-stimulated β-cells governing pulsatile insulin release. The glucose response of mouse β-cells is often manifested as slow oscillations and rapid transients of [Ca2+] i. LÄS MER

  5. 4. The interplay of ions in the stimulation of the pancreatic -cell

    Författare :Michael Eberhardson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell biology; diabetes; pancreatic β-cells; cytoplasmic calcium; oscillations; glucose; tetrodotoxin; tolbutamide; veratridine; tetraethylammonium; quinine; cesium; H2DIDS; furosemide; transients; microfluorometry; fura-2; MQAE; calcium channels; potassium channels; sodium channels; chloride channels; Cellbiologi; Cell biology; Cellbiologi; medicinsk cellbiologi; Medical Cell Biology;

    Sammanfattning : Glucose stimulation of insulin release is mediated by depolarisation of the pancreatic β-cells with accompanying entry of Ca2+ through voltage-dependent channels. An important feature of the glucose-induced depolarisation is its rhythmicity causing oscillations of the cytoplasmic Ca2+ concentration ([Ca2+]i), which trigger pulsatile release of insulin. LÄS MER

  7. 5. Endothelial Nitric Oxide in the Control of Glomerular Hemodynamics

    Författare :János Pittner; Daniel H. Casellas; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell biology; Juxtamedullary nephron; afferent arteriole; nitric oxide; endothelial calcium; diaminofluoresceins; fura-2; autoregulation; shear stress; flow; videomicroscopy; Cellbiologi; Cell biology; Cellbiologi; Physiology; fysiologi;

    Sammanfattning : The afferent arteriole of the kidney is a unique vascular segment: it is the effector of the renal hemodynamic autoregulation. The use of the isolated, perfused juxtamedullary nephron preparation makes it possible to visualize the afferent arteriole while preserving its tissue surroundings and the functional integrity of the nephron. LÄS MER