Sökning: "Free RNA polymerase"

Visar resultat 1 - 5 av 17 avhandlingar innehållade orden Free RNA polymerase.

  1. 1. The Importance of a Fifth Element in Transcription: Transcriptional Discrimination in Escherichia coli

    Författare :Bertil Gummesson; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; RNA polymerase; ppGpp; DksA; stringent response; passive regulation;

    Sammanfattning : Upon growth arrest of the bacterium Escherichia coli, RNA polymerase (Es70) is redirected from transcribing genes encoding the protein synthesizing system (PSS) to those involved in maintenance and stress resistance. The small nucleotide ppGpp, which is directly targeting Es70, is a key regulatory molecule required for this response. LÄS MER

  3. 2. Statistical processing of Flash X-ray Imaging of protein complexes

    Författare :Alberto Pietrini; Carl Nettelblad; Johan Bielecki; Janos Hajdu; Flavio Capotondi; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; X-ray; XFEL; single particle; hit-finder; statistical hit-finder; single protein; protein complex; RNA polymerase II; FEL; free-electron laser; FXI; Flash X-ray Imaging; CXI; CSPAD; x-ray imaging; COACS; Physics with specialization in Biophysics; Fysik med inriktning mot biofysik;

    Sammanfattning : Flash X-ray Imaging (FXI) at X-ray Free Electron Lasers (XFELs) is a promising technique that permits the investigation of the 3D structure of molecules without the need for crystallization, by diffracting on single individual sample particles.In the past few years, some success has been achieved by using FXI on quite large biological complexes (40 nm-1 μm in diameter size). LÄS MER

  4. 3. Structural and functional analyses of infectious pancreatic necrosis virus (IPNV)

    Författare :Anna Hjalmarsson; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cellular localisation; glycosylation; VP3-RNA cores; virus-specified components; immuno dot blot; TCID50; IPNV; purification; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : Infectious pancreatic necrosis virus (IPNV) is a naked virus of an uncomplicated structure. In the interior part of the virion, the two segments of double-stranded RNA are located together with the minor structural protein VP1. This protein is detected in its free form as well as linked to the genome. LÄS MER

  5. 4. The consequences of DNA lesions for mitochondrial DNA maintenance

    Författare :Josefin M. E. Forslund; Sjoerd Wanrooij; Nasim Sabouri; Erik Johansson; Claes Gustafsson; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mitochondria; mitochondrial DNA; DNA replication; DNA lesions; DNA polymerase γ; ribonucleotide incorporation; medicinsk biokemi; Medical Biochemistry;

    Sammanfattning : Eukaryotic cells have their own energy-producing organelles called mitochondria. The energy is stored in the adenosine triphosphate (ATP) molecule and is produced via the oxidative phosphorylation process inside the mitochondria. Thirteen of the essential proteins required for this process are encoded on the mitochondrial DNA (mtDNA). LÄS MER

  7. 5. Chromatin transcription and dietary proteins : with observations on the RNA synthesis of rat liver in response to the protein content and amino acid composition in the diet

    Författare :Stefan U. Åström; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : This thesis aims at characterization of fractionated chromatin with respect to bound and free RNA polymerase activities together with an analysis of the in vitro transcription of chromatin after changing the protein or amino acid content of the diet.Rat liver nuclei were incubated with micrococcal nuclease which acts as an endonuclease on DNA. LÄS MER