Sökning: "Flow Cytometry methods"

Visar resultat 1 - 5 av 111 avhandlingar innehållade orden Flow Cytometry methods.

1. 1. Flow cytometry for bioprocess control

   Författare :Fredrik Wållberg; KTH; []
   Nyckelord :flow cytometry; partec pas; propidium iodide;

   Sammanfattning : During bio-technical processing it is important to monitorbiological parameters such as cell growth, viability andproduct formation. Many of the analyses traditionally used areslow to perform and provide only average data for thepopulation. LÄS MER

3. 2. Acoustic Forces in Cytometry and Biomedical Applications: Multidimensional Acoustophoresis

   Författare :Carl Grenvall; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Microfluidics; Ultrasound; Multinode; Cell Sorting; Particle Positioning; Cytometry; Lab-on-a-Chip; µTAS; Binary Separation; Acoustic Barriers; Acoustic Particle Manipulation; Acoustic Standing Waves; Blood Separation; Milk Analysis;

   Sammanfattning : Over the last decades the ongoing work in the fields of Lab-on-a-Chip and Micro-Total-Analysis-Systems has led to the discovery of new or improved ways to handle and analyse small volumes of biofluids and complex biosuspensions. The benefits of working on the microscale include: miniaturization of the analysis systems with less need for large sample volumes; temporal and spatial control of suspended particle/cell positions; low volume sheath flow lamination or mixing; novel separation techniques by using forces inherent to the microscale domain; precise regulation of sample temperatures and rapid analysis with less volumes needed to be processed. LÄS MER

4. 3. Image-based quantitative infrared analysis and microparticle characterisation for pulp and paper applications

   Författare :Kari Hyll; Lars Mattsson; Hannes Vomhoff; Samuel Schabel; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Metrology; stock; papermaking; refining; fibrillation; fines; filler; morphology; classification; flow microscopy; fibre analyser; flow cytometry; laser diffraction; dynamic image analysis; process variation; thermography; emittance; emissivity; infrared; MWIR; LWIR; goniometer; Production Engineering; Industriell produktion; Fiber- och polymervetenskap; Fibre and Polymer Science;

   Sammanfattning : Measurements of process variations and particle morphology are widely employed in the pulp and paper industry. Two techniques with high potential, infrared thermography and microparticle characterisation, are mainly used qualitatively. LÄS MER

5. 4. Development of novel molecular methods for Campylobacter species and for Helicobacter pylori

   Författare :Kristina Blom; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Campylobacter; Helicobacter pylori; PCR; mRNA; hybridization; flow cytometry; HpaA; urease; NAP;

   Sammanfattning : Background and Aim: Different Campylobacter species and Helicobacter pylori are common causes of gastrointestinal infections that may result in diarrhea and chronic gastritis, respectively. The overall aim of this thesis was to develop novel methods for identification, differentiation and characterization of these bacteria. LÄS MER

7. 5. Improvements of the Bromodeoxyuridine-DNA Flow Cytometry Method for the Study of Cell Proliferation

   Författare :Maria C Johansson; Bröstcancer-genetik; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; head and neck cancer; [3H]thymidine; energy transfer; cell cycle; xenografted tumours; cultured cells; potential doubling time; labelling index; DNA synthesis time; cell proliferation; growth kinetics; Bromodeoxyuridine; DNA flow cytometry; cancer; onkologi; Cytologi; cancerology; oncology; Cytology;

   Sammanfattning : Potential doubling time (Tpot), DNA synthesis time (TS), and labelling index (LI) are fundamental growth kinetics parameters in clinical and experimental cancer research, which may be of further practical importance regarding prognosis and treatment prediction of cancer. They can be measured by bromodeoxyuridine(BrdUrd)/flow cytometry (FCM) methods, where BrdUrd, an analogue of thymidine, is incorporated into DNA and quantified simultaneously with the DNA content. LÄS MER