Sökning: "Enzyme Unfolding"

Visar resultat 1 - 5 av 13 avhandlingar innehållade orden Enzyme Unfolding.

  1. 1. Stability and inactivation mechanisms of two transaminases

    Författare :Shan Chen; Per Berglund; Patrick Adlercreutz; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Amine Transaminase; Operational Stability; Inactivation Pathway; Enzyme Unfolding; Phosphate Group Binding Cup; Bioteknologi; Biotechnology;

    Sammanfattning : In the past decades, more and more enzymes are employed as biocatalysts in industrial processes because of their advantages, such as high efficiency, substrate selectivity and stereoselectivity. Among them, amine transaminases (ATAs) are pyridoxal 5’-phosphate (PLP) dependent enzymes. LÄS MER

  3. 2. Angiopoietin-like protein 4 : an unfolding chaperone regulating lipoprotein lipase activity

    Författare :Valentina Sukonina; Gunilla Olivecrona; Lena Kjellen; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Lipoprotein lipase; angiopoietin-like protein 4; protein folding; human adipose tissue; macrophages; peroxisome proliferator-activated receptor delta PPARδ ; Medical microbiology; Medicinsk mikrobiologi;

    Sammanfattning : Lipoprotein lipase (LPL) is the main enzyme hydrolyzing triglyceride-rich lipoproteins in plasma. Proteoglycan-bound LPL on the vascular endothelium represent the functional pool of active enzyme. LPL is regulated in a tissue specific manner according to metabolic demands. LÄS MER

  4. 3. Computational Studies of the Temperature Dependence of Enzyme Catalysis

    Författare :Florian van der Ent; Johan Åqvist; Hugo Gutierrez de Teran; Nigel Richards; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : Enzymes are known to adapt to low temperatures by lowering the enthalpy of activation of the key reaction steps. A lesser known property of some psychrophilic (cold-active) enzymes is an anomalous temperature optimum. LÄS MER

  5. 4. Molecular development of a thermostable β-glucosidase for modification of natural products

    Författare :Sami Khan; Bioteknik; []
    Nyckelord :Thermotoga neapolitana; TnBgl1A; Mutagenesis; Flavonoid; Quercetin; Immobilisation; Chaperones;

    Sammanfattning : The gene encoding a β-glucosidase originating from the extreme thermophile Thermotoga neopolitana has been cloned and expressed in Escherichia coli. The aim was to produce a thermostable enzyme that could be used to remove sugar residues from glucosylated natural products classified as flavonoids by applying a method combining extraction in hot pressurized water with enzymatic hydrolysis. LÄS MER

  7. 5. A thermostable alkaline protease from a new alkaliphilic Nesterenkonia sp

    Författare :Shahrzad Bakhtiar; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; substrate specificity; crystallization; Biotechnology; Bioteknik; DNA-DNA hybridisation; 16S rDNA analysis; phenotypic characterisation; circular dichroism; differential scanning calorimetry; Nesterenkonia abyssinica; stability; calcium-independent; alkaline protease; alkaliphilic;

    Sammanfattning : This thesis concerns a novel alkaline protease produced by an extremophilic microbial specie, designated as AL20, isolated from a feather sample collected at the shore of the alkaline soda lake Abjata in the Ethiopian Rift Valley. The isolate is a heterotrophic, alkaliphilic, halotolerant gram-positive, strictly aerobic, non-motile, non-spore forming bacterium. LÄS MER