Sökning: "ENZYME ELECTROPHORESIS"
Visar resultat 1 - 5 av 31 avhandlingar innehållade orden ENZYME ELECTROPHORESIS.
1. Studies of immune responses to cell surface proteins of Helicobacter pylori and Borrelia burgdorferi by enzyme immunoassay and immunoblotting
Sammanfattning : Helicobacter pylori and Borrelia burgdorferi s.l. can cause chronic infections by evading the immune system. These two human pathogens express a number of immunogenic cell surface proteins. LÄS MER
2. Spectroscopic studies of biomolecules confined in self-assembled nanostructures
Sammanfattning : The present thesis examines the effects of incorporating biomolecules into self-assembled nanostructures. This approach is exemplified by short DNA molecules in environments close to lipid bilayers and the immobilization of enzymes into mesoporous silica particles. LÄS MER
3. Hormone-Sensitive Lipase, new roles for an old enzyme
Sammanfattning : Hormone-sensitive lipase (HSL) has a wide tissue distribution with the highest expression found in white adipose tissue (WAT). HSL also has a unique ability to hydrolyze a large panel of substrates including acylglycerols, cholesteryl esters and retinyl esters. HSL is potentially a new drug target for the treatment of obesity and type II diabetes. LÄS MER
4. Enantioselective Binding of Designed Propranolol Analogues to the Cellobiohydrolase Cel7A
Sammanfattning : This thesis describes the affinity and enantiomeric selectivity of the cellobiohydrolase Cel7A (isolated from Hypocrea jercorina). The chiral recognition of the enzyme has been studied with respect to amino alcohols and new ligands have been designed, based on the structure of the common ?-blocker propranolol. LÄS MER
5. Methodological studies of eicosanoid enzyme gene expression in vascular endothelium exposed to biomechanical stress
Sammanfattning : Prostaglandins are the major eicosanoids synthesized by vascular endothelial cells, and the vasculature is an important target organ for their bioactive metabolites. In vitro studies have shown that biomechanical forces are important regulators of vascular expression of prostanoid enzymes and release of metabolites. LÄS MER