Sökning: "Dipyridophenazine"

Visar resultat 1 - 5 av 8 avhandlingar innehållade ordet Dipyridophenazine.

  1. 1. Chemically Modified Oligonucleotides: Synthesis, Physicochemical and Biochemical Properties of their Duplexes with DNA and RNA

    Författare :Pushpangadan Indira Pradeepkumar; Jyoti Chattopadhyaya; Jesper Wengel; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; Antisense oligonucleotides; Dipyridophenazine; Oxetane; RNase H; Gene down-regulation; DNase 1; Charge transport; Bioorganisk kemi; Bioorganic chemistry; Bioorganisk kemi;

    Sammanfattning : This thesis is based on 9 papers dealing with the synthesis, physicochemical and biochemical properties of two types of chemically modified oligonucleotides which have the potential to down-regulate gene expression: (i) The first set is comprised of antisense oligonucleotides (AONs) conjugated with different chromophores of varying size, charge and π-electron density. Conjugation of the chromophores at the 3'- or 5'-end enhanced the target RNA binding affinity and RNase H recruitment capabilities compared to the native counterpart without changing the global helical conformation of their AON/RNA hybrid duplexes. LÄS MER

  3. 2. Retinoids and Ruthenium Complexes: Spectroscopic Probes for Lipid Membranes

    Författare :Frida Svensson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; retinoic acid; lipid vesicle; liposomes; dipyridophenazine; orientation; linear dichroism; membrane interaction; ruthenium; lipid chain ordering;

    Sammanfattning : .... LÄS MER

  4. 3. Polarized light spectroscopy studies of model membranes and DNA

    Författare :Maxim Kogan; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; emission; ruthenium dipyridophenazine complex; lipid bilayer; linear dichroism; membrane model; membrane orientation; bimolecular probe; DNA;

    Sammanfattning : Over the past decades Linear Dichroism (LD) spectroscopy has proven to be a powerful tool for studies of structure and functioning of different cell constituents, including DNA, cell membranes and proteins. Due to structural complexity of cell constituents, the LD studies of biomolecules depend on development of simplified model systems, which can mimic the original structure and functionality of the cell components. LÄS MER

  5. 4. Probing Biomolecules and Membranes using Spectroscopy and Microscopy

    Författare :Frida Svensson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ruthenium dipyridophenazine complex; biomolecular probes; microscopy; membrane; tryptophan; retinoic acid; peptide-lipid interaction; Linear dichroism; emission; lifetime imaging;

    Sammanfattning : .... LÄS MER

  7. 5. Cellular Dyes and Bioactive Peptides - Cell Membrane Interactions and Cellular Uptake

    Författare :Maria Matson Dzebo; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Membrane Interactions; Spectroscopy; Cell-Penetrating Peptides; Bioactive Peptides; Cell Membrane; Confocal Laser Scanning Microscopy; Cellular Uptake; AF-16; Voltage-Sensitive Dyes; Cell Dyes; Antisecretory Factor; Ruthenium Complexes; Isothermal Titration Calorimetry; Flow-Cytometry;

    Sammanfattning : Knowledge about mechanisms behind interactions of molecules with biomembranes and cellular uptake is very important for understanding biological processes and for drug design. The work described in this thesis has focused on interactions of cellular dyes and bioactive peptides with cells and the cell membrane. LÄS MER