Sökning: "Diethard Mattanovich"

Hittade 3 avhandlingar innehållade orden Diethard Mattanovich.

  1. 1. Methods for cell line and protein engineering

    Författare :Magnus Lundqvist; Johan Rockberg; Mathias Uhlén; Diethard Mattanovich; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell line development; therapeutic proteins; protein engineering; molecular cloning; mutagenesis; split-GFP; Bioteknologi; Biotechnology;

    Sammanfattning : Therapeutic proteins are becoming increasingly important. They are desirable, as they typically possess low adverse effects and higher specificity compared to the traditional, small molecule drugs. But they are also more complex and involve different intricate and expensive development and production processes. LÄS MER

  2. 2. Metabolic engineering and cultivation strategies for recombinant production of (R)-3-hydroxybutyrate

    Författare :Mariel Perez-Zabaleta; Gen Larsson; Antonius J. A. van Maris; Diethard Mattanovich; KTH; []
    Nyckelord :Escherichia coli; R -3-hydroxybutyrate; nitrogen limitation; nitrogen depletion; lignocellulose; fed batch; acetate; β-ketothiolase; acetoacetyl-CoA reductase; Halomonas boliviensis.; Bioteknologi; Biotechnology;

    Sammanfattning : Metabolic engineering and process engineering are two powerful disciplines to design and improve microbial processes for sustainable production of an extensive number of compounds ranging from chemicals to pharmaceuticals. The aim of this thesis was to synergistically combine these two disciplines to improve the production of a model chemical called (R)-3-hydroxybutyrate (3HB), which is a medium-value product with a stereocenter and two functional groups. LÄS MER

  3. 3. Proteome wide protein production

    Författare :Hanna Tegel; Sophia Hober; Diethard Mattanovich; KTH; []
    Nyckelord :protein production; Escherichia coli; transcription; promoter; translation; rare codon; high-throughput; screening;

    Sammanfattning : Over a decade after the completion of the human genome, researchers around the world are still wondering what information is hidden in the genome. Although the sequences of all human genes are known, it is still almost impossible to determine much more than the primary protein structure from the coding sequence of a gene. LÄS MER