Sökning: "Diagnostic PCR"

Visar resultat 1 - 5 av 152 avhandlingar innehållade orden Diagnostic PCR.

  1. 1. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

    Författare :Rickard Knutsson; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. LÄS MER

  3. 2. Tularemia : epidemiological, clinical and diagnostic aspects

    Författare :Henrik Eliasson; Erik Bäck; Anders Sjöstedt; Clas Ahlm; Örebro universitet; []
    Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; Francisella; tularemia; epidemiology; case-control; diagnosis; PCR; clinical characteristics; immunology; ELISA; lymphocyte stimulation; Medicine; Medicin; Infektionssjukdomar; Infectious Diseases;

    Sammanfattning : Tularemia is a zoonosis caused by the small, fastidious, gram-negative rod Francisella tularensis that appears over almost the entire Northern Hemisphere. In Sweden, tularemia has appeared mainly in restricted areas in northern parts of central Sweden. LÄS MER

  4. 3. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

    Författare :Amal Elfaitouri; Jonas Blomberg; Helene Norder; Uppsala universitet; []
    Nyckelord :Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

    Sammanfattning : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. LÄS MER

  5. 4. Mammographic density. A marker of treatment outcome in breast cancer?

    Författare :Ida Dalene Skarping; Bröstcancer - prevention & intervention; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mammographic density; pCR; Neoadjuvant chemotherapy; Diagnostic precision;

    Sammanfattning : .... LÄS MER

  7. 5. Optimisation of diagnostic PCR a study of PCR inhibitors in blood and sample pretreatment

    Författare :Waleed Abu Al-Soud; Avdelningen för medicinsk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; mycology; virology; bacteriology; Microbiology; AmpliTaq Gold; Amplification facilitators; Betaine; Blood culture medium; Blood; BSA; cheese; faeces; gp32; Haemoglobin; Ions; Immunoglobulin G; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : PCR is widely employed as a rapid, sensitive and specific molecular diagnostic technique in clinical diagnosis, environmental investigations and for monitoring biotechnical processes. However, the full potential of diagnostic PCR is limited, in part, by the presence of PCR-inhibitory substances in biological samples. LÄS MER