Sökning: "Deep sequencing"
Visar resultat 16 - 20 av 68 avhandlingar innehållade orden Deep sequencing.
16. Metabolic Engineering of Recombinant Protein Production by Saccharomyces cerevisiae
Sammanfattning : The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and it also provides a platform for the production of many heterologous proteins of medical or industrial interest. In this thesis, random and rational approaches, such as vector design, host engineering, fermentation analysis, UV Mutation, coupled with high-throughput systems biology techniques (including whole genomic sequencing, microarray analysis and flux analysis) and integrated analysis (Reporter feature technique), were employed to engineer cellular properties more effectively and purposefully to construct cell factories for protein production. LÄS MER
17. Phenotypic antibody discovery and mining of complex antibody libraries
Sammanfattning : Therapeutic antibodies are the fastest growing class of drugs but suffer from a crowded target-space where many antibodies, against a few targets, are developed in parallel. The aim of this thesis is to find methods to enable discovery of novel antibody-target combinations and thereby increase the target-space. LÄS MER
18. Metabolic Engineering of Central Carbon Metabolism in Saccharomyces cerevisiae The contribution of systems biology to physiological studies
Sammanfattning : Saccharomyces cerevisiae is one of the most well characterized yeast of large industrial interest due to several attractivefeatures such as its capability to efficiently convert glucose into ethanol and carbon dioxide at high flux, its amenabilityto genetic modification and the presence of extensive knowledge databases. For these reasons, it is often considered asuitable cell factory for the production of different classes of compounds. LÄS MER
19. The deep evolutionary roots of non-coding RNA - a comparative genomics approach
Sammanfattning : Non-coding RNAs (ncRNA) are a diverse group of genes that do not encode proteins but function exclusively on the level of RNA and were originally suggested to be remnants of a pre-DNA stage of life known as the RNA world. More recent work, however, has uncovered a rich repertoire of previously unknown families with possible consequences for our understanding of the origin and evolution of the modern RNA infrastructure. LÄS MER
20. Massively parallel analysis of cells and nucleic acids
Sammanfattning : Recent proceedings in biotechnology have enabled completely new avenues in life science research to be explored. By allowing increased parallelization an ever-increasing complexity of cell samples or experiments can be investigated in shorter time and at a lower cost. LÄS MER
