Sökning: "DNA uptake"

Visar resultat 1 - 5 av 128 avhandlingar innehållade orden DNA uptake.

  1. 1. Condensation of DNA for Gene Delivery: Studies on Vector-Nucleic Acid Interactions and Transfection Efficiency

    Författare :Kristina Fant; Chalmers University of Technology; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; dendrimer; endocytosis; uptake; gene delivery; optical spectroscopy; structure-activity relationship; DNA; cell-penetrating peptide;

    Sammanfattning : This thesis concerns the delivery of nucleic acids into cells and tissues with the aim to change or correct gene expression. The field of gene delivery has the ultimate goal of producing genetic drugs for clinical applications, and the activity in this area is intense although so far the use is limited to research purposes. LÄS MER

  2. 2. Cell-Penetrating Penetratin Peptides - Mechanistic Studies on Uptake Pathways and DNA Delivery Efficiency

    Författare :Helene Åmand; Chalmers University of Technology; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Plasma membrane vesicle; Lysine; Stimulated endocytosis; Gene delivery; Cell surface affinity; Penetratin; Cell-penetrating peptide; Cysteine; Uptake; Arginine;

    Sammanfattning : Delivery of gene-targeted drugs is limited by the inherently poor capacity of nucleic acids to overcome the membrane barrier of the cell, and development of vectors that can promote uptake is therefore crucial. Cell penetrating peptides (CPPs) have emerged as promising vector candidates due to their ability to deliver a wide range of macromolecular cargos into cells. LÄS MER

  3. 3. The Cyanobacterial Uptake Hydrogenase : Regulation, Maturation and Function

    Författare :Marie Holmqvist; Peter Lindblad; Karin Stensjö; Kornél Kovács; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Biohydrogen; CalA; Cyanobacteria; Hydrogenase; Maturation; Nostoc punctiforme ATCC 29133; Nostoc sp. PCC 7120; Transcriptional regulation; Molecular biology; Molekylärbiologi; Molecular biology; Molekylärbiologi; Chemistry with specialization in Microbial Chemistry; Kemi med inriktning mot mikrobiell kemi;

    Sammanfattning : With accellerating global warming and pollution problems a change of energy regime is necessary. Solar energy offers a clean and unlimited energy source of enormous potential. Due to it’s intermittenet nature solar energy must be stored - ideally in the chemical bond of a carrier molecule. LÄS MER

  4. 4. Endocytosis of therapeutic macromolecules in tumor cells - Mechanistic aspects of the proteoglycan receptor function

    Författare :Anders Wittrup; Bröstcancer-genetik; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; LL-37; heparan sulfate; glypican; endocytosis; drug delivery; DNA uptake; Cancer; cell penetrating peptides; non-viral gene delivery; organelle purification; proteoglycan; syndecan.;

    Sammanfattning : Novel therapeutics to combat cancer are urgently needed. Most current pharmacological therapies have severe side effects and are seldom curative. Macromolecular drugs, and in particular nucleic acid based drugs, offer a potential remedy for this situation. LÄS MER

  5. 5. Optical Dyes for Visualization of Structure and Function of Biomolecules and Biological Membranes

    Författare :Maria Matson; Chalmers University of Technology; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Orientation; RNA; Lipid Membranes; Voltage-Sensitive Dyes; Binding Preferences; Cellular Localization; DNA; Spectroscopy; Spectral Properties; Lipophilic Ruthenium Complex;

    Sammanfattning : To visualize the structure and function of biomolecules and biological membranes in the cell, optical dyes are important tools where in particular fluorescent probes provide a sensitive and selective detection. When cellular compartments are investigated it is crucial that the probe selectively binds to the area of interest and not exhibits unspecific binding affinity. LÄS MER