Sökning: "DNA substrate"

Visar resultat 1 - 5 av 174 avhandlingar innehållade orden DNA substrate.

  1. 1. Structural and dynamical basis of NusA´s role in transcription-coupled DNA repair

    Författare :Damasus Okeke; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Resonance assignment; DNA repair; transcription-repair coupling factors; transcription-coupled repair; NusA; DinB; UvrD; autoinhibition; DNA substrate; dynamics.;

    Sammanfattning : DNA repair processes involves a host of repair machineries that are able to recognize and repair the damaged DNA. Nucleotide excision repair (NER) pathway is one of these processes exploited by cells to remove bulky DNA lesions. LÄS MER

  3. 2. Biophysical studies of DNA binding – by the large filament-forming protein Rad51 and the small minor-groove binder Hoechst 33258

    Författare :Louise Fornander; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Rad51; Linear Dichroism; DNA; Isothermal Titration Calorimetry.; Spectroscopy; Nanofluidics; Hoechst 33258; DNA-binding ligands;

    Sammanfattning : Mechanistic insight into the nature of DNA-binding ligands is crucial for both drug development as well as understanding more complex biological reactions that take place in the cell. In this Thesis, two rather different DNA-binding molecules are considered: 1. LÄS MER

  4. 3. Electric DNA arrays for determination of pathogenic Bacillus cereus

    Författare :Yanling Liu; Sven-Olof Enfors; Helena Anderson; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bacillus cereus; haemolysin; non-haemolytic enterotoxin; cytotoxin K-2; cereulide; toxin-coding genes; bacterial colony; electric DNA chip; ultrasonication; DNA fragmentation; Biochemistry; Biokemi;

    Sammanfattning : Silicon-based electric chip arrays were developed for characterization of Bacillus cereus with respect to the capacity to produce toxins involved in food poisoning and foodborne infections. Bacteria of the B. cereus group contain different sets of four toxins encoded by eight genes. LÄS MER

  5. 4. A thermostable alkaline protease from a new alkaliphilic Nesterenkonia sp

    Författare :Shahrzad Bakhtiar; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; substrate specificity; crystallization; Biotechnology; Bioteknik; DNA-DNA hybridisation; 16S rDNA analysis; phenotypic characterisation; circular dichroism; differential scanning calorimetry; Nesterenkonia abyssinica; stability; calcium-independent; alkaline protease; alkaliphilic;

    Sammanfattning : This thesis concerns a novel alkaline protease produced by an extremophilic microbial specie, designated as AL20, isolated from a feather sample collected at the shore of the alkaline soda lake Abjata in the Ethiopian Rift Valley. The isolate is a heterotrophic, alkaliphilic, halotolerant gram-positive, strictly aerobic, non-motile, non-spore forming bacterium. LÄS MER

  7. 5. Silicon nanowire based devices for More than Moore Applications

    Författare :Ganesh Jayakumar; Per-Erik Hellström; Mikael Östling; Luca Selmi; KTH; []
    Nyckelord :silicon nanowire; biosensor; CMOS; sequential integration; lab-on-chip; LOC; high-K; high-K integration on SiNW biosensor; ALD; fluid gate; back gate; SiNW; SiNW pixel matrix; FEOL; pattern transfer lithography; sidewall transfer lithography; STL; multi-target bio detection; BEOL; nanonets; silicon nanonets; SiNN-FET; SiNW-FET; CMOS integration of nanowires; CMOS integration of nanonets; monolithic 3D integration of nanowires; above-IC integration of nanowires; DNA detection using SiNW; SiNW biosensor; dry environment DNA detection; DNA hybridization detection using SiNW; SiNW functionalization; SiNW silanization; SiNW grafting; FEOL integration of SiNW; BEOL integration of SiNW; sequential multiplexed biodetection; biodetection efficiency of SiNW; front end of line integration of SiNW; back end of line integration of SiNW; SiNW dry environment functionalization; APTES cross-linker; accessing SiNW test site; fluorescence microscopy of SiNW; geometry of SiNW; SiNW biosensor variability; top-down fabrication of SiNW; bottom-up fabrication of SiNW; VLS method; ams foundry CMOS process; adding functionality in BEOL process; sensor integration in BEOL process; hafnium oxide; HfO2; aluminium oxide; Al2O3; TiN backgate; Nickel source drain; ISFET; ion sensitive field effect transistor; Overcoming Nernst limit of detection using SiNW; SiNW sub-threshold region operation; ASIC; SOC; SiGe selective epitaxy; epitaxial growth of SiNW; epitaxial growth of nanowires; epitaxial growth of nanonets; nickel silicide contacts; salicide process; high yield SiNW fabrication; high volume SiNW fabrication; silicon ribbon; SiRi pixel; SiRi biosensor; SiRi DNA detection; monolithic 3D integration of nanonets; above-IC integration of nanonets; impact of back gate voltage on silicon nanowire; impact of back gate voltage on SiNW; FDSOI; fully depleted silicon on insulator technology; metal backgate; wafer scale integration of SiNW; wafer scale integration of nanonets; impact of backgate voltage on CMOS inverter circuit; frequency divider; D flip-flop; Informations- och kommunikationsteknik; Information and Communication Technology;

    Sammanfattning : Silicon nanowires (SiNW) are in the spotlight for a few years in the research community as a good candidate for biosensing applications. This is attributed to their small dimensions in nanometer scale that offers high sensitivity, label-free detection and at the same time utilizing small amount of sample. LÄS MER