Sökning: "DNA purification"

Visar resultat 1 - 5 av 85 avhandlingar innehållade orden DNA purification.

  1. 1. Strategies for de novo DNA sequencing

    Författare :Anna Blomstergren; KTH; []
    Nyckelord :DNA sequencing; DNA purification; automation; solid-phase; streptavidin; biotin; modular probes; Helicobacter pylori; cag PAI;

    Sammanfattning : The development of improved sequencing technologies hasenabled the field of genomics to evolve. Handling andsequencing of large numbers of samples require an increasedlevel of automation in order to obtain high throughput andconsistent quality. LÄS MER

  3. 2. Advancing DNA-based proximity methods

    Författare :Mattias Leino; Ola Söderberg; John Löfblom; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; in situ; proximity dependent intiation of hybridization chain reaction; hybridization chain reaction; Oligonucleotide design; proximity ligation assay; protein interaction; microscopy; purification; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : Cellular functions are governed by intricate chains of interactions between proteins. In order to properly understand cellular biology one must look into, not only protein function, but also its interacting network. LÄS MER

  4. 3. DNA Analysis of PCR-Inhibitory Forensic Samples

    Författare :Johannes Hedman; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; amylase; statistical modelling; principal components analysis; PCR inhibitors; PCR inhibition; forensic DNA analysis; DNA polymerase blend; DNA polymerase; crime scene samples;

    Sammanfattning : DNA evidence, linking perpetrators to crime scenes, is central to many legal proceedings. However, crime scene samples often contain extraneous substances that may interfere with the PCR-based forensic analysis, resulting in partial or negative DNA profiles. Extensive DNA purification may remove inhibitors, but involves the risk of DNA loss. LÄS MER

  5. 4. DNA vaccines and bacterial DNA in immunity

    Författare :Lisa Charlotta Bandholtz; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :DNA vaccine; CpG DNA; antimicrobial peptides C. pneumoniae; Shigella spp;

    Sammanfattning : This thesis describes DNA-based vaccination and the importance of bacterial DNA in different immunological perspectives. Intranasal (i.n.) DNA vaccination utilizing a plasmid encoding the chlamydial heat shock protein 60 (p-hsp-60) generated lower bacterial burden and reduced pathology in the lungs of mice after subsequent infection with C. LÄS MER

  7. 5. Functional and structural properties of eukaryotic DNA polymerase epsilon

    Författare :Olga Chilkova; Erik Johansson; Per Elias; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; eukaryotic DNA replication; DNA polymerase epsilon; protein-DNA interaction; Biochemistry; Biokemi;

    Sammanfattning : In eukaryotes there are three DNA polymerases which are essential for the replication of chromosomal DNA: DNA polymerase alpha (Pol alpha), DNA polymerase delta (Pol delta) and DNA polymerase epsilon (Pol epsilon). In vitro studies of viral DNA replication showed that Pol alpha and Pol delta are sufficient for DNA replication on both leading and lagging DNA strands, thus leaving the function of Pol epsilon unknown. LÄS MER