Sökning: "DNA polymerases"

Visar resultat 1 - 5 av 35 avhandlingar innehållade orden DNA polymerases.

  1. 1. The consequences of DNA lesions for mitochondrial DNA maintenance

    Författare :Josefin M. E. Forslund; Sjoerd Wanrooij; Nasim Sabouri; Erik Johansson; Claes Gustafsson; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mitochondria; mitochondrial DNA; DNA replication; DNA lesions; DNA polymerase γ; ribonucleotide incorporation; medicinsk biokemi; Medical Biochemistry;

    Sammanfattning : Eukaryotic cells have their own energy-producing organelles called mitochondria. The energy is stored in the adenosine triphosphate (ATP) molecule and is produced via the oxidative phosphorylation process inside the mitochondria. Thirteen of the essential proteins required for this process are encoded on the mitochondrial DNA (mtDNA). LÄS MER

  3. 2. Ribonucleotides in DNA - Application in genome-wide DNA polymerase tracking and physiological role in eukaryotes

    Författare :Katrin Kreisel; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Ribonucleotides; DNA instability; DNA polymerase eta; nuclear DNA; mitochondrial DNA;

    Sammanfattning : The genetic code in the eukaryotic cell is stored in the form of DNA, which is more resistant to hydrolysis than RNA. Replication fidelity and DNA repair mechanisms are in place to ensure genomic integrity to preserve the information encoded. LÄS MER

  4. 3. DNA Analysis of PCR-Inhibitory Forensic Samples

    Författare :Johannes Hedman; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; amylase; statistical modelling; principal components analysis; PCR inhibitors; PCR inhibition; forensic DNA analysis; DNA polymerase blend; DNA polymerase; crime scene samples;

    Sammanfattning : DNA evidence, linking perpetrators to crime scenes, is central to many legal proceedings. However, crime scene samples often contain extraneous substances that may interfere with the PCR-based forensic analysis, resulting in partial or negative DNA profiles. Extensive DNA purification may remove inhibitors, but involves the risk of DNA loss. LÄS MER

  5. 4. Structure of eukaryotic DNA polymerase epsilon and lesion bypass capability

    Författare :Nasim Sabouri; Erik Johansson; Lars Thelander; Ola Hammarsten; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA polymerase epsilon; DNA replication; Okazaki fragment; Translesion synthesis; DNA lesion; dNTP; Biochemistry; Biokemi;

    Sammanfattning : To transfer the information in the genome from mother cell to daughter cell, the DNA replication must be carried out only once and with very high fidelity prior to every cell division. In yeast there are several different DNA polymerases involved in DNA replication and/or DNA repair. LÄS MER

  7. 5. Role of yeast DNA polymerase epsilon during DNA replication

    Författare :Isabelle Isoz; Erik Johansson; Juhani Syväoja; Umeå universitet; []
    Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; DNA polymerase epsilon; eukaryotic DNA replication; fidelity; leading strand; Dpb2; Biochemistry and clinical chemistry; Biokemi och klinisk kemi;

    Sammanfattning : Each cell division, the nuclear DNA must be replicated efficiently and with high accuracy to avoid mutations which can have an effect on cell function. There are three replicative DNA polymerases essential for the synthesis of DNA during replication in eukaryotic cells. LÄS MER