Sökning: "DNA polymerase"

Visar resultat 21 - 25 av 255 avhandlingar innehållade orden DNA polymerase.

  1. 21. Structural and biochemical basis for the high fidelity and processivity of DNA polymerase ε

    Författare :Rais Ahmad Ganai; Erik Johansson; Per Elias; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; DNA polymerase ε; Crystal structure; P domain; Pre-steady state kinetics; β-hairpin loop; Primer switching; Strand displacement; biokemi; Biochemistry;

    Sammanfattning : DNA polymerase epsilon (Pol ε) is a multi-subunit B-family DNA polymerase that is involved in leading strand DNA replication in eukaryotes. DNA Pol ε in yeast consists of four subunits, Pol2, Dpb2, Dpb3, and Dpb4. Pol2 is the catalytic subunit and Dpb2, Dpb3, and Dpb4 are the accessory subunits. LÄS MER

  3. 22. Herpes Simplex Virus 1 DNA replication and its role in recombination and transcription

    Författare :Ka-Wei Tang; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Herpes Simplex Virus 1; DNA replication; DNA recombination; Transcription;

    Sammanfattning : Herpes simplex virus 1 (HSV-1) is one of nine different herpesvirus infecting man. They are all capable of establishing a life-long latent state following the primary infection. HSV-1 as well as other herpesviruses may reactivate from the latent state and give rise to a productive infection with clinical symptoms or asymptomatic shedding. LÄS MER

  4. 23. Initiation of Mammalian Mitochondrial DNA Replication

    Författare :Elisabeth Jemt; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mitochondria; mtDNA; DNA replication;

    Sammanfattning : Mitochondria produce most of the adenosine triphosphate required in a eukaryotic cell and they contain their own genome. The mitochondrial DNA (mtDNA) is a double stranded circular molecule that codes for proteins required for cellular respiration and RNA molecules involved in translation of these proteins. LÄS MER

  5. 24. Protein Engineering by Directed Evolution and Rational Design

    Författare :Torbjörn Gräslund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ion-exchange chromatography; subtilisin; Klenow DNA polymerase; Taq DNA polymerase; directed evolution; rational design; charge engineering; phosphonylating inhibitor; phage display; circular dichroism; protein A; Bioengineering; Bioteknik;

    Sammanfattning : .... LÄS MER

  7. 25. Microfluidic bead-based methods for DNA analysis

    Författare :Aman Russom; Ulf Gyllensten; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; single nucleotide polymorphism; DNA analysis; SNP; microfluidics; pyrosequencing; beads; lab on a chip; hybridization; DASH; microsystem; micro totat analysis system; allele-specific extension; DASH; microcontact printing; Genetik; Clinical genetics; Klinisk genetik;

    Sammanfattning : With the completion of the human genome sequencing project, attention is currently shifting toward understanding how genetic variation, such as single nucleotide polymorphism (SNP), leads to disease. To identify, understand, and control biological mechanisms of living organisms, the enormous amounts of accumulated sequence information must be coupled to faster, cheaper, and more powerful technologies for DNA, RNA, and protein analysis. LÄS MER