Sökning: "DNA polymerase"

Visar resultat 11 - 15 av 255 avhandlingar innehållade orden DNA polymerase.

  1. 11. AtZDP, a Plant 3' DNA Phosphatase, Involved in DNA Repair

    Författare :Isabel Valsecchi; Lars Rask; Per Jemth; Christer Larsson; Uppsala universitet; []
    Nyckelord :Molecular biology; AtZDP; plant long patch Base Excision Repair; DNA 3 phosphate blocking lesions; DNA repair; Molekylärbiologi;

    Sammanfattning : DNA bases can be modified by endogenous agents (e.g. oxidized by products of respiration and photosynthesis or methylated by gene silencing processes) as well as by environmental agents (e.g. LÄS MER

  3. 12. DNA precursor biosynthesis-allosteric regulation and medical applications

    Författare :Reza Rofougaran; Anders Hofer; Thomas Borén; Staffan Eriksson; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; DNA biosynthesis; ribonucleotide reductase; allosteric regulation; Trypanosoma brucei; adenosine kinase; nucleoside analogs; mitochondrial DNA; TWINKLE; Biokemi; medicinsk biokemi; Medical Biochemistry;

    Sammanfattning : Ribonucleotide reductase (RNR) is a key enzyme for de novo dNTP biosynthesis. We have studied nucleotide-dependent oligomerization of the allosterically regulated mammalian RNR using a mass spectrometry–related technique called Gas-phase Electrophoretic Mobility Macromolecule Analysis (GEMMA). LÄS MER

  4. 13. Organization of centromere associated DNA in Chironomus pallidivittatus

    Författare :Hong He; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Genetics; Chironomus.; pericentric DNA; repetitive DNA; centromere; transposable element; cytogenetics; Genetik; cytogenetik;

    Sammanfattning : Centromeres have not yet been functionally defined in higher eu- karyotes, largely due to the huge amounts of repeated DNA in these regions of most species and difficulties of delimiting centromeric from pericentric regions. Also the latter have large quantities of mostly noncoding DNA, expressed cytologically as pericentric heterochromatin. LÄS MER

  5. 14. Engineering Surfaces of Solid-State Nanopores for Biomolecule Sensing

    Författare :Shiyu Li; Shi-Li Zhang; Michael Mayer; Uppsala universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; solid-state nanopores; surface engineering; DNA sensing; DNA polymerase; optical detection; lipid bilayer; hafnium oxide; electron beam induced deposition;

    Sammanfattning : Nanopores have emerged as a special class of single-molecule analytical tool that offers immense potential for sensing and characterizing biomolecules such as nucleic acids and proteins. As an alternative to biological nanopores, solid-state nanopores present remarkable versatility due to their wide-range tunability in pore geometry and dimension as well as their excellent mechanical robustness and stability. LÄS MER

  7. 15. Recombinant Enzymes in Pyrosequencing Technology

    Författare :Nader Nourizad; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

    Sammanfattning : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. LÄS MER