Sökning: "DNA melting"

Visar resultat 1 - 5 av 24 avhandlingar innehållade orden DNA melting.

  1. 1. Statistical Physics of DNA: Melting and Confinement Effects

    Författare :Michaela Reiter-Schad; Beräkningsbiologi och biologisk fysik - Genomgår omorganisation; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; biopolymers; DNA melting; DNA confinement; random walk; Fysicumarkivet A:2015:Reiter-Scad;

    Sammanfattning : Investigation of single macromolecules makes it possible to gain insight into and characterize important biological processes. DNA as the molecule of life is of special interest here. LÄS MER

  3. 2. Fluorescence Probing of DNA Structures in Biology and Nanotechnology

    Författare :Peter Sandin; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; Fluorescent DNA base analog; UV‐Vis spectroscopy; DNA nanostructure; self‐assembly; time‐resolved fluorescence spectroscopy;

    Sammanfattning : Much has been learnt about DNA since its structure was discovered about half a century ago. Scientists everlasting urge to look beyond and deeper into the intricate machinery of life, has continuously pushed technology forward. LÄS MER

  4. 3. Overstretching short DNA – Single-molecule force spectroscopy studies

    Författare :Niklas Bosaeus; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA stretching; mechanical properties of DNA; single molecule; optical tweezers; DNA structure;

    Sammanfattning : Structural deformation of DNA has a central role in many biological processes. It occurs, for example, during replication, transcription, and regulation of the activity of the genome. To understand these fundamental processes it is necessary to have a detailed knowledge of the mechanical properties of DNA. LÄS MER

  5. 4. DNA strand exchange and hydrophobic interactions between biomolecules

    Författare :Bobo Feng; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; ATP synthase; DNA; membrane proteins; linear dichroism; Hydrophobic interactions; self-assembly; strand exchange; nanotechnology; liposomes;

    Sammanfattning : The role of hydrophobic interactions in DNA strand exchange has been studied using fluorescence-labeled DNA oligomers in a FRET assay. Strand exchange was found to be accelerated in the presence of polyethylene glycol, which provides a crowded and hydrophobic environment possibly mimicking that of the catalytically active recombinase-DNA complexes. LÄS MER

  7. 5. Nucleic Acids as Drug Targets -Interactions of Platinum Complexes, Polyamines, and siRNA with DNA and RNA Models

    Författare :Margareta Hägerlöf; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Kinetics; Kemi; Chemistry; AU-rich region; siRNA; Circular Dichroism; Polyamine; Modified Nucleoside; HPLC; Thermodynamic Stability; cisplatin; Hairpin; DNA and RNA conformation; RNA; DNA; Anticancer Drug; Platinum Complexes;

    Sammanfattning : Drugs targeting nucleic acids were studied in DNA and RNA model systems. The effects were investigated with respect to structural and thermodynamic influence, kinetics, and biological function. The drugs employed in this thesis were anticancer active platinum(II) complexes, polyamine derivatives, and siRNA. LÄS MER