Sökning: "DNA hybridization"

Visar resultat 1 - 5 av 172 avhandlingar innehållade orden DNA hybridization.

  1. 1. Advances in DNA Detection

    Författare :Yajing Song; Olof Emanuelsson; Jonas Tegenfeldt; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA detection; active filter paper; visual detection; throughput; fluorescence; superparamagnetic beads; rare DNA; Q-PCR; chemical fragmentation; DNA library preparation; massively parallel sequencing; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : DNA detection technologies have an increasing importance in our everyday lives, with applications ranging from microbial diagnostics to forensic analysis, food safety evaluation, and environmental monitoring. Currently, as the associated costs decrease, DNA diagnostic techniques are routinely used in research laboratories, in clinical and forensic practice. LÄS MER

  3. 2. Concatemerization of Synthetic Oligonucleotides

    Författare :Lu Sun; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; gel electrophoresis; DNA-binding; QCM-D; DNA concatemer; hybridization; DNA formation; self-assembly;

    Sammanfattning : DNA nanotechnology has become an important research field because of its advantages in high predictability and accuracy of base-paring recognition. For example, the DNA concatemer, one of the simplest DNA constructs in shape, has been used to enhance signals in biosensing. LÄS MER

  4. 3. Concatemerization of Synthetic Oligonucleotides, Assembly on Surface and Ligand Interactions

    Författare :Lu Sun; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA formation; DNA concatemer; self-assemble; gel electrophoresis; DNA-binding; hybridization; QCM-D;

    Sammanfattning : DNA nanotechnology has become an important research field because of its advantages in high predictability and accuracy of base-paring recognition. DNA concatemer, one of the simplest DNA constructs in shape, have been found as efficient signal amplifiers in several biosensors for different applications. LÄS MER

  5. 4. Formation and Characterization of DNA Concatemers for Single Molecule Mechanism Studies

    Författare :Lu Sun; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; concatemers; hybridization; DNA; gel electrophoresis; self-assembly; constructs; nanotechnology;

    Sammanfattning : Well known as genetic information carrier, DNA however, in the past three decade experienced a revolution to become a fashionable fabrication material in nanotechnology as a consequence of the high predictability and accuracy of base-paring recognition. Although DNA can be constructed in many fancy 2D and 3D structures, this thesis work focused on constructing one-dimension linear DNA concatemers in order to study sequence specific DNA-ligand binding at the single-molecule level. LÄS MER

  7. 5. Digital Electrical DNA Sensing

    Författare :Maoxiang Guo; Wouter van der Wijngaart; Fredrik Westerlund; KTH; []
    Nyckelord :DNA detection; thiol-ene-epoxy; rolling circle amplification; digital measurement; DNA stretching; microwell-array; gold nanowires; electrical measurement; specificity; limit of detection; liquid storage; disease diagnosis; lab-on-a-chip; point-of-care.;

    Sammanfattning : Molecule detection is a workhorse in life sciences and medicine, for example in cancer diagnosis and virus and bacterial detection. DNA analysis can provide vital information about the state of a host organism and its medical and health condition. LÄS MER