Sökning: "DNA detection methods"

Visar resultat 6 - 10 av 200 avhandlingar innehållade orden DNA detection methods.

1. 6. Methods for barcode analysis in optical DNA mapping

   Författare :Albertas Dvirnas; Beräkningsbiologi och biologisk fysik - Genomgår omorganisation; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA Barcoding; Optical Mapping; Computational Biology;

   Sammanfattning : This thesis is composed of six papers, which all concern different methods and tools used for the analysis of barcodes in nanochannel-based Optical DNA Mapping (ODM). The first four papers consider densely-labeled barcodes while the last two consider sparsely-labeled barcodes. LÄS MER

3. 7. Competitive-Binding Based Optical DNA Mapping - From Bacterial Plasmids to the Human Genome

   Författare :Vilhelm Müller; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; genetics; single-molecule; nanofluidics; DNA; antibiotic resistance; optical DNA mapping; plasmids; competitive binding; fluorescence microscopy; bacteria;

   Sammanfattning : Significant advances within the field of DNA sequencing have allowed us to study DNA at a level of detail that was previously impossible. However, dynamic genomic regions with a high degree of structural variations, while being linked to disease in humans and increased resistance to antibiotics, are still challenging to characterize. LÄS MER

4. 8. Visual and magnetic detection of antimicrobial resistance genes using nanoparticles and isothermal amplification

   Författare :Darío Sánchez Martín; Teresa Zardán Gómez de la Torre; Maria Strömme; Mahiar Hamedi; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA amplification; isothermal amplification; visual detection; nanoparticles; VAM-NDA; rolling circle amplification; phi29 DNA polymerase; padlock probes.; Teknisk fysik med inriktning mot nanoteknologi och funktionella material; Engineering Science with specialization in Nanotechnology and Functional Materials;

   Sammanfattning : Antibiotic resistance is becoming an increasing concern, necessitating new ways to diagnose and treat it rapidly and accurately. One proposed solution is the use of nucleic acid amplification tests, capable of detecting resistance in just a few hours, as opposed to the several days that traditional testing methods may require. LÄS MER

5. 9. Single Molecule Detection : Microfluidic Automation and Digital Quantification

   Författare :Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

   Sammanfattning : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. LÄS MER

7. 10. Methods for deep examination of DNA

   Författare :Mårten Neiman; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :massive parallel sequencing;

   Sammanfattning : The development of sequencing technology has had a rapid pace during the last years and today, the sequencing instruments harbors enormous capacity. This thesis is about the development of methods to make the most out of this capacity and to use it for various applications. LÄS MER