Sökning: "DNA detection methods"

Visar resultat 21 - 25 av 171 avhandlingar innehållade orden DNA detection methods.

  1. 21. Optical Mapping of Bacterial Plasmids

    Författare :Vilhelm Müller; Chalmers University of Technology; []
    Nyckelord :NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; competitive binding; optical mapping; fluorescence microscopy; DNA; plasmids; CRISPR Cas9; antibiotic resistance; nanofluidics; single-molecule;

    Sammanfattning : Bacteria that have acquired resistance to antibiotics represent one of the largest threats to human health and modern health care. The genes encoding resistance are frequently spread via the transfer of plasmids, which are circular double stranded DNA molecules separate from the chromosomal DNA of the bacteria. LÄS MER

  2. 22. Molecular Tools for Biomarker Detection

    Författare :Lei Chen; Ulf Landegren; Niko Hildebrandt; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Rolling circle amplification; padlock probe;

    Sammanfattning : The advance of biological research promotes the emerging of new methods and solutions to answer the biological questions. This thesis describes several new molecular tools and their applications for the detection of genomic and proteomic information with extremely high sensitivity and specificity or simplify such detection procedures without compromising the performance. LÄS MER

  3. 23. Correlation Between Phenotype and Genotype in Some Clinically Important Blood group Systems

    Författare :Nidal Irshaid; Avdelningen för hematologi och transfusionsmedicin; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Immunologi; transplantation; serology; transfusion medicine; Blood group; DNA; Immunology; genomic typing; serologi;

    Sammanfattning : In the past decade, the molecular basis for most of the blood group systems has been investigated intensively. Many blood group genes have been cloned and the polymorphisms of clinically significant blood group systems have been elucidated. LÄS MER

  4. 24. Development of DNA-based methods for analysis of protein interactions

    Författare :Axel Klaesson; Ola Söderberg; Mats Nilsson; Cristina Al-Khalili Szigyarto; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICAL AND HEALTH SCIENCES; In situ proximity ligation assay PLA ; rolling circle amplification RCA ; Hybridization chain reaction HCR ; proxHCR; Oligonucleotide design; Protein-protein interactions; Post-translational modifications.; Farmaceutisk vetenskap; Pharmaceutical Science; Molekylär cellbiologi; Molecular Cellbiology;

    Sammanfattning : In situ proximity ligation assay (PLA) is a method for detection of protein interactions, post-translational modifications (PTMs) and individual proteins that allows information about their localization in a cell or tissue to be extracted. The method is based on oligonucleotide-conjugated antibodies (proximity probes) that upon binding of two epitopes in close proximity give rise to an amplifiable DNA circle. LÄS MER

  5. 25. Debugging metabarcoding for insect biodiversity studies

    Författare :Daniel Marquina Hernández; Fredrik Ronquist; Owen Wangensteen; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; metabarcoding; insects; non-destructive; DNA extraction; Malaise trap; preservative ethanol; environmental DNA; Systematic Zoology; systematisk zoologi;

    Sammanfattning : Insects are one of the most abundant and diverse animal groups, and they include many valuable ecological indicator species, but taxonomic discovery projects and biodiversity surveys targeting this group are often challenging. While mass trapping devices allow the collection of insects in great numbers, the task of identifying the species present is a painstaking and resource-demanding process. LÄS MER