Sökning: "DNA detection methods"

Visar resultat 16 - 20 av 171 avhandlingar innehållade orden DNA detection methods.

1. 16. Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders

   Författare :Simon Fredriksson; Larry Gold; Uppsala universitet; []
   Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; SELEX; antibody; DNA microarray; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molecular Medicine; molekylär medicin genetik och patologi ;

   Sammanfattning : A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. LÄS MER

3. 17. Bayesian inference for detection problems in biology

   Författare :Ronny Hedell; Göteborgs universitet; Göteborgs universitet; Gothenburg University; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PCR; Bayesian inference; Forensic DNA analysis; Syndromic surveillance; Bacillus anthracis; Markov chain Monte Carlo; Allele dropout; Bacillus anthracis;

   Sammanfattning : This thesis is about different kinds of detection problems in biology: detection of DNA sequences in crime scene samples, detection of harmful bacteria in feed and food stuff and detection of epidemical diseases in animal populations. In each case, biological data is produced or collected in order to determine which DNA sequences, bacteria types or diseases are present, if any. LÄS MER

4. 18. PCR-based detection of microorganisms in complex biological samples

   Författare :Pär-G Lantz; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Density gradient centrifugation; DNA extraction; Aqueous two-phase systems; Meat; Faeces; Cheese; Yeast; Helicobacter pylori; Yersinia enterocolitica; Listeria monocytogenes; PCR; Inhibition; Sample preparation; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

   Sammanfattning : The detection of microorganisms by PCR can be divided into four steps: (1) sample collection, (2) sample preparation, (3) DNA amplification and (4) detection of PCR products. The major problem in developing PCR-based detection methods is the sample preparation step. LÄS MER

5. 19. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

   Författare :Amal Elfaitouri; Jonas Blomberg; Helene Norder; Uppsala universitet; []
   Nyckelord :Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

   Sammanfattning : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. LÄS MER

7. 20. Single-molecule Detection in situ

   Författare :Chatarina Larsson; Mats Nilsson; Ulf Landegren; Sanjay Tyagi; Uppsala universitet; []
   Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Molekylär medicin; Molecular Medicine; molekylär medicin genetik och patologi ;

   Sammanfattning : The human body contains a variety of different cell types that share a common genome, but differ in how they use the information encoded therein. Variation in molecular content exists even at the level of individual cells, and to provide deeper insight into complex cellular processes methods that permit analysis of each cell on its own are needed. LÄS MER