Sökning: "DNA analogs"

Visar resultat 1 - 5 av 39 avhandlingar innehållade orden DNA analogs.

  1. 1. Fluorescence Probing of DNA Structures in Biology and Nanotechnology

    Författare :Peter Sandin; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; Fluorescent DNA base analog; UV‐Vis spectroscopy; DNA nanostructure; self‐assembly; time‐resolved fluorescence spectroscopy;

    Sammanfattning : Much has been learnt about DNA since its structure was discovered about half a century ago. Scientists everlasting urge to look beyond and deeper into the intricate machinery of life, has continuously pushed technology forward. LÄS MER

  3. 2. DNA precursor biosynthesis-allosteric regulation and medical applications

    Författare :Reza Rofougaran; Anders Hofer; Thomas Borén; Staffan Eriksson; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; DNA biosynthesis; ribonucleotide reductase; allosteric regulation; Trypanosoma brucei; adenosine kinase; nucleoside analogs; mitochondrial DNA; TWINKLE; Biokemi; medicinsk biokemi; Medical Biochemistry;

    Sammanfattning : Ribonucleotide reductase (RNR) is a key enzyme for de novo dNTP biosynthesis. We have studied nucleotide-dependent oligomerization of the allosterically regulated mammalian RNR using a mass spectrometry–related technique called Gas-phase Electrophoretic Mobility Macromolecule Analysis (GEMMA). LÄS MER

  4. 3. Fluorescent DNA Modifications for Use as Probes of Biological Structure and as Nanotechnological Functional Units

    Författare :Karl Börjesson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; FRET; nanotechnology; DNA; DNA base analogs; fluorescence; porphyrin; lipid membrane;

    Sammanfattning : .... LÄS MER

  5. 4. Intelligent Nucleic Acid Interactions with Peptide Nucleic Acid (PNA) and Recombination Enzyme RecA

    Författare :Pernilla Wittung; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fluorescence; RecA; homologous recombination; circular dichroism; DNA analogs; -anti-BPDE; spectroscopy; PNA; DNA-protein interactions;

    Sammanfattning : The work presented in this thesis focuses on interactions between nucleic-acids in biological systems, particularly interactions of the promising drug-candidate peptide nucleic acid (PNA), and on protein-DNA intermediates during recombination. Several experimental techniques, mainly circular dichroism and fluorescence spectroscopy, have been used to characterize the interactions of interest. LÄS MER

  7. 5. Model Systems for Metal - DNA Interactions. With a Focus on Anticancer Active Platinum(II) Complexes

    Författare :Anna Ericson; Kemiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; kinetics; DNA; poly d T ; guanine; 6-thioinosine-5 -monophosphate; 6-thio-2 -deoxyinosine; 4-thiouridine-5 -monophosphate; 4-thio-2 -deoxyuridine; palladium II ; Gold III ; platinum II ; mechanism; salt effect; activation parameters; Inorganic chemistry; Oorganisk kemi;

    Sammanfattning : Reactions of trans-dicyanodichloroaurate(III) trans-dibromodicyanoaurate(III), trans-diamminedichlorogold(III), cis-diammine(aqua)chloroplatinum(II), cis-ammineaqua-(cyclohexylamine)chloroplatinum(II), chloro(terpyridine)platinum(II) and chloro-(diethylenetriaminepalldium(II) with dimethyl sulfide, thionucleosides, thionucleotides and thione- and guanine-containing 17-mer oligonucleotides have been investigated by use of conventional and high-pressure stopped-flow spectrophotometry and HPLC technique as a function of ionic strength, pH, temperature and pressure in aqueous solutions. The mechanism and rate of oxidation of dimethyl sulfide by gold(III) complexes depends on the concentration of free halide. LÄS MER