Sökning: "DHAP"

Hittade 5 avhandlingar innehållade ordet DHAP.

  1. 1. Improved techniques for CE and MALDI-MS including microfluidic hyphenations foranalysis of biomolecules

    Författare :Johan Jacksén; Åsa Emmer; Tomas Bergman; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides proteins; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon micro canal; Matrix; Peptide-fluorescein isothiocyanate adducts; Bovine serum albumin; DHAP; Hyphenations; Hydrophobicity; Single wood fiber analysis; Pre-concentration; Concentration platform; Analytical chemistry; Analytisk kemi;

    Sammanfattning : In this thesis, improved techniques for biomolecule analysis using capillary electrophoresis (CE) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and hyphenations between those have been presented.A pre-concentration method which is possible to apply in both techniques, has also been investigated. LÄS MER

  3. 2. Improved techniques for CE-MALDI-MS off-line coupling and MALDI-MS analysis of primarily hydrophobic proteins and peptides

    Författare :Johan Jacksén; Åsa Emmer; Anna Karin/Minna Borg Karlsson/Hakkarainen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon microcanal; Matrix; Protein cleavage digestion.; Kapillärelektrofores; Hydrofoba peptider; Matris-assisterad laserdesorptions-joniserings-masspektrometri; Strukturbelagd provplatta; Off-line interface; Mikrokanal i kisel; Matris; Proteinklyvning -spjälkning.; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Due to the hydrophobic nature of integral membrane proteins (IMP) they give rise to several difficulties concerning handling and analysis, which is not the case for the most water soluble proteins. New analysis methods are needed, where the insolubility problems of the hydrophobic proteins due to aggregation and adhesion are tackled. LÄS MER

  4. 3. The GRE3 encoded aldo-keto reductase and its influence on xylose fermentation in recombinant Saccharomyces cerevisiae strains

    Författare :Karin Träff; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biochemical technology; Biokemisk teknik; dihydroxyacetone phosphate; xylose reductase; xylose; xylose isomerase; GRE3; Saccharomyces cerevisiae; aldo-keto reductase;

    Sammanfattning : The aim of the work presented in this thesis was to improve ethanol formation from xylose in recombinant Saccharomyces cerevisiae strains. This was achieved by decreasing the xylitol by-product formation. Gre3p is the major xylose-reducing enzyme in S. cerevisiae. LÄS MER

  5. 4. Functional studies of the TDH and GPD gene-products in the glycolytic flux and glycerol metabolism of Saccharomyces cerevisiae

    Författare :Hadi Valadi; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Growth rate; GPD; Sacchromyces cerevisiae; Glyceol; NADH; Anaerobiosis; TDH; Redox; Glycolysis;

    Sammanfattning : This thesis focuses on the physiology and control of the glycolysis and glycerol metabolism in the yeast Sacchromyces cerevisiae. The study is based on the relation ship between the first two reactions around the branch-point of the upper and lower part of glycolysis and the glycerol pathway. LÄS MER

  7. 5. Temporal monitoring of intracellular Ca2+ signaling and origins of Ca2+ oscillations

    Författare :Dominic-Luc Webb; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : This thesis examined parameters influencing stimulated cytoplasmic free Ca2+ concentration ([Ca2+]i) oscillations in hepatocytes and pancreatic beta-cells. Hepatic glucose output is regulated in part by hormones such as vasopressin that act through [Ca 2+]i oscillations. LÄS MER