Sökning: "Combinatorial antibody libraries"

Visar resultat 1 - 5 av 13 avhandlingar innehållade orden Combinatorial antibody libraries.

  1. 1. Engineering Antibody Specificity Using Combinatorial Antibody Libraries

    Författare :Jenny Boström; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; antibody Antigen Interactions; Dual Specific Antibodies; Herceptin; Human Epidermal Growth Factor Receptor 2; Thermodynamic Analysis; therapeutic Antibody Engineering; Synthetic Phagedisplayed Antibody Libraries; Vascular Endothelial Growth Factor; Bacterial Display; trastuzumab;

    Sammanfattning : The research presented in this thesis investigates antibody specificity, antibody-antigen and protein-protein interactions using combinatorial antibody libraries, phage-display and bacterial display technology. One goal was to develop a method to generate antibodies that bind two protein antigens with high affinity using a single-antigen binding site, and to investigate the molecular and thermodynamic requirements for antibody multispecificity. LÄS MER

  3. 2. Combinatorial Methodology. Screening for affinity specific ligands to biological and artificial receptors

    Författare :Margareta Krook; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; peptide library; combinatorial; screening; phage display; affinity; DNA-interaction; chymotrypsin; peptide inhibitor; antibody; IgG; Fc; molecular imprinting; steroid; receptor; Biochemistry; Metabolism; Biokemi; Biotechnology; Bioteknik;

    Sammanfattning : Combinatorial screening methodology has been employed to select new affinity specific ligands to bilogical and artificial receptors. The screening methodology, in particular screening of combinatorial phage display peptide libraries against target molecules differing in function and shape, is focused upon. LÄS MER

  4. 3. Engineering of small IgG binding domains for antibody labelling and purification

    Författare :Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Bioteknologi; Biotechnology;

    Sammanfattning : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. LÄS MER

  5. 4. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization

    Författare :Francis Jingxin Hu; Johan Rockberg; John McCafferty; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody engineering; antibody affinity maturation; combinatorial protein engineering; epitope mapping; FACS; HER2; complement C5; Staphylococcal surface display; surface display.; Bioteknologi; Biotechnology;

    Sammanfattning : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. LÄS MER

  7. 5. Affibody ligands in immunotechnology applications

    Författare :Jenny Rönnmark; KTH; []
    Nyckelord :phage display; combinatorial; affinity; IgA ligand; immunohistochemistry; affibody-fusions;

    Sammanfattning : This thesis describes the development and use ofnon-immunoglobulin affinity proteins denoted affibodies asalternatives to antibodies in different immunotechnologyapplications. A 58 aa IgG Fc binding three-helix bundle domainZ, derived from staphylococcal protein A has been used asframework for library constructions, in which the face of themolecule involved in the native binding activity has beenengineered by combinatorial protein engineering. LÄS MER