Sökning: "Chromobacterium violaceum"

Visar resultat 1 - 5 av 10 avhandlingar innehållade orden Chromobacterium violaceum.

  1. 1. Transaminase Biocatalysis: Applications and Fundamental Studies

    Författare :Federica Ruggieri; Per Berglund; Mariarita Bertoldi; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biocatalysis; enzymatic cascade; transaminase; amine; racemization; PLP; enzyme engineering; Chromobacterium violaceum; protein stability; enzyme stability; molecular dynamics; structural biology; enzyme technology; chiral amines; kinetic resolution; Bioteknologi; Biotechnology;

    Sammanfattning : Biocatalysis is the branch of science at the intersection between chemistry and biology and specifcally dedicated to the application of natural evolvable catalysts, i.e. enzymes, in human-designed chemical processes. Among the array of promising biocatalysts, transaminases (EC 2. LÄS MER

  2. 2. Amine Transaminases in Biocatalytic Amine Synthesis

    Författare :Henrik Land; Per Berglund; Helen C. Hailes; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Amine Transaminase; Biocatalysis; Transamination; Reductive Amination; Enzyme; Enzyme Engineering; Equilibrium Displacement; Screening; Enzyme Stability; Bioteknologi; Biotechnology;

    Sammanfattning : The use of enzymes, nature´s own catalysts, both isolated or as whole cells to perform chemical transformations is called biocatalysis. As a complement to classical chemical catalysis, biocatalysis can be an environmentally friendly and more economical option in the production and synthesis of chemicals. LÄS MER

  3. 3. ω-Transaminase in Biocatalysis : Methods, Reactions and Engineering

    Författare :Karim Engelmark Cassimjee; Per Berglund; Wofgang Kroutil; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : Biocatalysis offers an alternative to classic chemistry by using enzymes, the protein catalysts of Nature, for production of fine chemicals. Evolution has created enzymes capable of catalysis at moderate temperature of a specific reaction in the presence of a plethora of compounds in the aqueous cell environment. LÄS MER

  4. 4. Quantum Chemical Studies of Enzymatic Reaction Mechanisms : Investigations of Cytosine Deaminase and ω-Transaminase

    Författare :Bianca Manta; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : In this thesis, density functional theory is used to study the reaction mechanisms of two dierent enzymes. Quantum chemical cluster models of the active sites were designed using available crystal structures. LÄS MER

  5. 5. Stability and inactivation mechanisms of two transaminases

    Författare :Shan Chen; Per Berglund; Patrick Adlercreutz; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Amine Transaminase; Operational Stability; Inactivation Pathway; Enzyme Unfolding; Phosphate Group Binding Cup; Bioteknologi; Biotechnology;

    Sammanfattning : In the past decades, more and more enzymes are employed as biocatalysts in industrial processes because of their advantages, such as high efficiency, substrate selectivity and stereoselectivity. Among them, amine transaminases (ATAs) are pyridoxal 5’-phosphate (PLP) dependent enzymes. LÄS MER