Sökning: "Christian Spahn"

Hittade 1 avhandling innehållade orden Christian Spahn.

1. 1. Choice of tRNA on Translating Ribosomes

   Författare :Elli Bouakaz; Måns Ehrenberg; Christian Spahn; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Ribosome; Translation; Accuracy; Proofreading; tRNA; Molekylärbiologi; Molecular biology; Molekylärbiologi;

   Sammanfattning : This thesis addresses different aspects of the question about accuracy of protein synthesis: i) the mechanism of tRNA selection during translation ii) study of ribosomal mutations that affect accuracy and iii) the choice of aminoacyl-tRNA isoacceptors on synonymous codons.By measuring the codon reading efficiencies of cognate and near-cognate ternary complexes we demonstrate that in optimal physiological conditions accuracy of substrate selection is much higher than previously reported; that during translation the ribosomal A site is not blocked by unspecific binding of the non-cognate tRNAs which would inhibit the speed of protein synthesis. LÄS MER