Sökning: "Chinese hamster ovary"

Visar resultat 1 - 5 av 32 avhandlingar innehållade orden Chinese hamster ovary.

  1. 1. Macroscopic models of Chinese hamster ovary cell cultures

    Författare :Erika Hagrot; Véronique Chotteau; Anders Forsgren; Nathan E. Lewis; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Chinese hamster ovary; Amino acid; Metabolic network; Metabolic flux analysis; Kinetic modeling; Elementary flux mode; Optimization; Column generation; Bioteknologi; Biotechnology;

    Sammanfattning : Biopharmaceuticals treat a range of diseases, and is a growing sector within the pharmaceutical industry. A majority of these complex molecules are produced by genetically modified mammalian cells in large-scale cell cultures. LÄS MER

  3. 2. High cell density perfusion process development for antibody producing Chinese Hamster Ovary cells

    Författare :Ye Zhang; Vincent Bulone; Veronique Chotteau; Carl-Fredrik Mandenius; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; WAVE Bioreactor; Cellbag; ATF; TFF; hollow fiber; CSPR; CellTank; SUB; single-use-bioreactor; disposable; perfusion rate; Biotechnology; Bioteknologi;

    Sammanfattning : Perfusion operation mode is currently under fast expansion in mammalian cell based manufacturing of biopharmaceuticals, not only for labile drug protein but also for stable proteins such as monoclonal antibodies (mAbs). Perfusion mode can advantageously offer a stable cell environment, long-term production with high productivity and consistent product quality. LÄS MER

  4. 3. Tools for the development of intensified perfusion processes for mammalian cell culture

    Författare :Hubert Schwarz; Véronique Chotteau; Mark Smales; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinant protein biomanufacturing; Perfusion process; Integrated continuous bioprocessing; Monoclonal antibody; Erythropoietin; Chinese Hamster Ovary cells; Human Embryonic Kidney cells; Perfusion medium optimization; Cell specific perfusion rate; Raman spectroscopy; N-glycosylation; Microbioreactors; Design of Experiments; Biotechnology; Bioteknologi;

    Sammanfattning : Recombinant protein therapeutics have become an indispensable part of modern medicine to treat a wide variety of diseases. Their manufacture is mostly based on mammalian cells with fed-batch production bioreactors and downstream batch purification unit operations. LÄS MER

  5. 4. Development of mathematical modelling for the glycosylation of IgG in CHO cell cultures

    Författare :Liang Zhang; Véronique Chotteau; Michael J. Betenbaugh; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Chinese hamster ovary cells; glycosylation; IgG; mathematical modelling; experimental design; perfusion; carbon sources; GReBA; Bayesian network; probabilistic graphic model; Bioteknologi; Biotechnology;

    Sammanfattning : Chinese hamster ovary (CHO) cells are the most popular expression system for the production of biopharmaceuticals. More than 80% of the approved monoclonal antibodies (mAbs) or immunoglobulin G (IgG) are produced with these cells. Glycosylation is a usual post- translational modification important for therapeutic mAbs. LÄS MER

  7. 5. Macroscopic Modeling of Metabolic Reaction Networks and Dynamic Identification of Elementary Flux Modes by Column Generation

    Författare :Hildur Æsa Oddsdóttir; Anders Forsgren; Bernhard Palsson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolic Network; Optimization; Robust Optimization; Least-squares; Column Generation; Modeling; Algorithm; Elementary Flux Mode; Metabolic Flux Analysis; Chinese Hamster Ovary Cell; Amino Acid Metabolism; Tillämpad matematik och beräkningsmatematik; Applied and Computational Mathematics;

    Sammanfattning : In this work an intersection between optimization methods and animal cell culture modeling is considered. We present optimization based methods for analyzing and building models of cell culture; models that could be used when designing the environment cells are cultivated in, i.e., medium. LÄS MER