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1. 1. Cellulose hydrolysis by Trichoderma reesei cellulases: Studies on adsorption, sugar production and synergism of cellobiohydrolase I, II and endoglucanase II

   Författare :Jozsef Medve; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; FPLC; cellulose hydrolysis; Trichoderma reesei; cellulase; cellobiohydrolase; endoglucanase; adsorption isotherm; synergism; enzyme purification; isoenzymes; quantitative analysis; Biochemistry; Metabolism; Biokemi;

   Sammanfattning : Three major cellulases, cellobiohydrolase I and II (CBH I and II) and endoglucanase II (EG II) of Trichoderma reesei have been purified by ion-exchange chromatography in an FPLC system. Microcrystalline cellulose (Avicel) was hydrolysed by the single enzymes and by equimolar mixtures of CBH I-CBH II and CBH I-EG II. LÄS MER

3. 2. The kinetics of cellulose enzymatic hydrolysis : Implications of the synergism between enzymes

   Författare :Priit Väljamäe; Jan-Christer Janson; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Acetobacter; Cellobiohydrolase; Cellobiose; Cellulase; Cellulose; Diffusion; Endoglucanase; Hydrolysis; Inhibition; Kinetics; Model; Product; Substrate; Surface; Synergism; Trichoderma reesei; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

   Sammanfattning : The hydrolysis kinetics of bacterial cellulose and its derivatives by Trichoderma reesei cellulases was studied. The cellulose surface erosion model was introduced to explain the gradual and strong retardation of the rate of enzymatic hydrolysis of cellulose. LÄS MER

4. 3. Enantioselective Binding of Designed Propranolol Analogues to the Cellobiohydrolase Cel7A

   Författare :Alexandra Fagerström; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Strukturkemi; Structure chemistry; enzymologi; Proteiner; enzymology; Proteins; Naturvetenskap; Natural science; capillary electrophoresis; chiral recognition; enantiomeric selectivity; cellobiohydrolase Cel7A; propranolol; Organic chemistry; Organisk kemi;

   Sammanfattning : This thesis describes the affinity and enantiomeric selectivity of the cellobiohydrolase Cel7A (isolated from Hypocrea jercorina). The chiral recognition of the enzyme has been studied with respect to amino alcohols and new ligands have been designed, based on the structure of the common ?-blocker propranolol. LÄS MER

5. 4. Degradation of wood polysaccharides by fungal glycoside hydrolases

   Författare :Torny Eriksson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Penicillium brasilianum; ethanol; cellulose; wood polysaccharides; Man5A hemicellulase; cellulase; endoglucanase; cellobiohydrolase; Cel7B; Cel7A; Trichoderma reesei; glycoside hydrolase; Plant biochemistry; Växtbiokemi;

   Sammanfattning : The enzymatic degradation of wood polysaccharides such as cellulose and hemicellulose is an important process in nature. In addition, cellulases and hemicellulases can be used in industrial applications. Fuel ethanol can potentially be produced from wood by enzymatic hydrolysis of cellulose followed by yeast fermentation of the formed sugars. LÄS MER

7. 5. Aspects of Optimisation of Separation of Drugs by Chemometrics

   Författare :Valérie Harang; Douglas Westerlund; Sven P. Jacobsson; Bo Karlberg; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical chemistry; optimisation; separation; chemometrics; high performance liquid chromatography; electrodriven techniques; microemulsion electrokinetic chromatography; statistical experimental design; molecular modelling; chiral separation; cellobiohydrolase; Farmaceutisk kemi; Pharmaceutical chemistry; Farmaceutisk kemi;

   Sammanfattning : Statistical experimental designs have been used for method development and optimisation of separation. Two reversed phase HPLC methods were optimised. LÄS MER